Sarker N, Tsudzuki M, Nishibori M, Yamamoto Y
Laboratory of Animal Breeding and Genetics, Faculty of Applied Biological Science, Hiroshima University, Kagamiyama, Higashi-Hiroshima, Japan.
Poult Sci. 1999 Jan;78(1):1-7. doi: 10.1093/ps/78.1.1.
Two pairs of chicken lines were divergently selected for serum immunoglobulin M and G levels at 10 wk of age to examine the effect of selection on direct and correlated traits. After three generations of selection, the lines were tested for their ability of antibody production against SRBC, a T cell dependent antigen. At 14 and 17 wk of age, the lines were immunized intravenously with 1 mL of 5% SRBC solution. Blood samples were collected at 7 and 14 d of post primary immunization (PPI) and post secondary immunization (PSI). Selection was effective (P < 0.01) in changing the serum immunoglobulin M levels. The G levels were changed in a downward direction initially in the high line. The differences between the high immunoglobulin M (HIM) and low immunoglobulin M (LIM) lines as well as high immunoglobulin G (HIG) and low immunoglobulin G (LIG) lines became larger during the course of selection. Selection differentials varied considerably during the selection, but the selection response was almost constant. Realized heritability was relatively high, ranging from 0.58 to 0.66 and from 0.40 to 0.60 for the IgM and IgG selected lines, respectively. Total antibody titers to SRBC differed significantly (P < 0.01) between the HIM and LIM lines at 7 and 14 d PPI, and 7 d PSI. The HIM line also produced significantly higher mercaptoethanol resistant antibody titers to SRBC than the LIM line at 7 and 14 d PPI and PSI. The LIG line had significantly higher total antibody titers to SRBC than the HIG line at both 7 and 14 d of PPI and PSI. From these results, it is suggested that selection of chickens on the basis of serum immunoglobulin isotypes may change antibody producing cells as well as other immunocompetent cells that modulate the immune response of the selected lines.
选取两对鸡品系，根据10周龄时血清免疫球蛋白M和G水平进行 divergent 选择，以研究选择对直接性状和相关性状的影响。经过三代选择后，检测这些品系针对T细胞依赖性抗原——绵羊红细胞（SRBC）产生抗体的能力。在14周龄和17周龄时，给这些品系静脉注射1 mL 5% SRBC溶液。在初次免疫后（PPI）和二次免疫后（PSI）的第7天和第14天采集血样。选择在改变血清免疫球蛋白M水平方面是有效的（P < 0.01）。高选系中免疫球蛋白G水平最初呈下降趋势。在选择过程中，高免疫球蛋白M（HIM）品系和低免疫球蛋白M（LIM）品系以及高免疫球蛋白G（HIG）品系和低免疫球蛋白G（LIG）品系之间的差异变得更大。选择差在选择过程中变化很大，但选择反应几乎是恒定的。实现的遗传力相对较高，IgM选择品系和IgG选择品系的遗传力分别为0.58至0.66和0.40至0.60。在PPI的第7天和第14天以及PSI的第7天，HIM品系和LIM品系之间针对SRBC的总抗体滴度差异显著（P < 0.01）。在PPI和PSI的第7天和第14天，HIM品系针对SRBC产生的巯基乙醇抗性抗体滴度也显著高于LIM品系。在PPI和PSI 的第7天和第14天，LIG品系针对SRBC的总抗体滴度均显著高于HIG品系。从这些结果表明，基于血清免疫球蛋白同种型选择鸡可能会改变产生抗体的细胞以及其他调节所选品系免疫反应的免疫活性细胞。
