Baelmans R, Parmentier H K, Nieuwland M G B, Dorny P, Demey F, Berkvens D
Department of Animal Sciences, Adaptation Physiology Group, Wageningen University, Marijkeweg 40, 6709 PG Wageningen, The Netherlands.
Trop Anim Health Prod. 2005 Apr;37(3):173-86. doi: 10.1023/b:trop.0000049274.28640.d7.
A total of 376 chickens from different ecotypes were immunized with the non-pathogenic multi-determinant antigen sheep red blood cells (SRBC). The ecotypes included indigenous chickens from various locations in Tanzania (n=102), India (n=86) and Bolivia (n=89). In addition, eight German Dahlem Red (GDR) chicken lines with different major genes (dwarf, naked neck and frizzled) of tropical interest were also immunized with SRBC. Immune competence of the breeds was assessed by measuring complement haemolytic activity, both from the classical calcium-dependent complement pathway (CPW) and alternative calcium-independent complement pathway (APW), alongside IgTotal, IgG and IgM antibody responses to SRBC at 7 days post immunization. Large variations in complement activity and antibody responses to SRBC were observed within and between the indigenous breeds. Many indigenous chickens, especially from Bolivia, showed decreased complement activity (APW) following immunization with SRBC. Breeds from India showed the highest CPW activity and humoral (especially IgM) responses to SRBC, suggesting high immune competence. In contrast, Bolivian chickens were characterized by low CPW activity, low APW activity and low antibody levels to SRBC suggesting an overall low immune competence. In the GDR chickens, characterized by high CPW activity and high IgG antibody responses to SRBC, the major genes for naked neck, frizzling and dwarfism had no significant effect on the antibody responses and complement activity to SRBC.
用非致病性多决定簇抗原绵羊红细胞（SRBC）对来自不同生态型的376只鸡进行免疫。这些生态型包括来自坦桑尼亚不同地点的本地鸡（n = 102）、印度的本地鸡（n = 86）和玻利维亚的本地鸡（n = 89）。此外，还对8个具有热带地区感兴趣的不同主要基因（矮小、裸颈和卷羽）的德国达勒姆红鸡品系用SRBC进行了免疫。通过测量经典钙依赖补体途径（CPW）和替代钙非依赖补体途径（APW）的补体溶血活性，以及免疫后7天对SRBC的总免疫球蛋白、IgG和IgM抗体反应，评估这些品种的免疫能力。在本地品种内部和之间观察到补体活性和对SRBC的抗体反应存在很大差异。许多本地鸡，特别是来自玻利维亚的，在用SRBC免疫后补体活性（APW）降低。来自印度的品种对SRBC表现出最高的CPW活性和体液（特别是IgM）反应，表明免疫能力高。相比之下，玻利维亚鸡的特点是CPW活性低、APW活性低以及对SRBC的抗体水平低，表明总体免疫能力低。在以对SRBC具有高CPW活性和高IgG抗体反应为特征的德国达勒姆红鸡中，裸颈、卷羽和矮小的主要基因对SRBC的抗体反应和补体活性没有显著影响。
