染色质重塑复合物介导的组蛋白八聚体转移

Histone octamer transfer by a chromatin-remodeling complex.

作者信息

Lorch Y, Zhang M, Kornberg R D

机构信息

Department of Structural Biology, Stanford University School of Medicine, California 94305, USA.

出版信息

Cell. 1999 Feb 5;96(3):389-92. doi: 10.1016/s0092-8674(00)80551-6.

DOI:10.1016/s0092-8674(00)80551-6

PMID:10025404

Abstract

RSC, an abundant, essential chromatin-remodeling complex related to SWI/SNF complex, catalyzes the transfer of a histone octamer from a nucleosome core particle to naked DNA. The newly formed octamer-DNA complex is identical with a nucleosome in all respects. The reaction requires ATP and involves an activated RSC-nucleosome intermediate. The mechanism may entail formation of a duplex displacement loop on the nucleosome, facilitating the entry of exogeneous DNA and the release of the endogenous molecule.

摘要

RSC是一种与SWI/SNF复合物相关的丰富且必需的染色质重塑复合物，它催化组蛋白八聚体从核小体核心颗粒转移到裸露的DNA上。新形成的八聚体-DNA复合物在各方面都与核小体相同。该反应需要ATP，并涉及一个活化的RSC-核小体中间体。其机制可能需要在核小体上形成双链置换环，以促进外源DNA的进入和内源分子的释放。