Ushida Y, Sekine K, Kuhara T, Takasuka N, Iigo M, Tsuda H
Experimental Pathology and Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan.
Cancer Lett. 1998 Dec 25;134(2):141-5. doi: 10.1016/s0304-3835(98)00249-3.
Chemopreventive effects of bovine lactoferrin (bLF), previously shown to strongly inhibit intestinal carcinogenesis in rats (K. Sekine, E. Watanabe, J. Nakamura, N. Takasuka, D.J. Kim, M. Asamoto, V. Krutovskikh, T.H. Baba, T. Ota, M.A. Moore, M. Masuda, H. Sugimoto, H. Nishino, T. Kakizoe, H. Tsuda, Inhibition of azoxymethane-initiated colon tumor by bovine lactoferrin administration in F344 rats, Jpn. J. Cancer Res. 88 (1997) 523-526; K. Sekine, Y. Ushida, T. Kuhara, M. Iigo, H. Baba-Toriyama, M.A. Moore, M. Murakoshi, Y. Satomi, H. Nishino, T. Kakizoe, H. Tsuda, Inhibition of initiation and early stage development of aberrant crypt foci and enhanced natural killer activity in male rats administered bovine lactoferrin concomitantly with azoxymethane, Cancer Lett. 121 (1997) 211-216), on spontaneous intestinal polyp development were assessed in the ApcMin mouse, a model for both familial adenomatous polyposis and sporadic colon cancers. In the experiment, 54 mice at 6 weeks of age were given 2% bLF (15 mice), 0.2% bLF (15 mice) and AIN-93G (24 mice) as basal diet ad libitum for 8 weeks. An overall tendency for a reduction in the total number of polyps in the small intestine was evident in the bLF-treated animals, along with significant suppression in the jejunum at the 2% dose (P < 0.05, 68% of the control). In addition, body growth suppression, presumed to be due to anemia and/or intussusception as a consequence of numerous polyps in the intestine, was alleviated. No toxic effects were observed in the intestinal epithelium. Although not as obvious as observed for the rat case, the data suggest that bLF may be a chemopreventor of intestinal polyposis.
牛乳铁蛋白（bLF）先前已被证明能强烈抑制大鼠肠道癌变（K. 关根、E. 渡边、J. 中村、N. 高须贺、D.J. 金、M. 浅本、V. 克鲁托夫斯基、T.H. 巴巴、T. 太田、M.A. 摩尔、M. 增田、H. 杉本、H. 西野、T. 柿添、H. 津田，在F344大鼠中给予牛乳铁蛋白对乙氧甲叉甲烷引发的结肠肿瘤的抑制作用，《日本癌症研究杂志》88 (1997) 523 - 526；K. 关根、Y. 内田、T. 久原、M. 饭尾、H. 巴巴 - 鸟山、M.A. 摩尔、M. 村越、Y. 里美、H. 西野、T. 柿添、H. 津田，在同时给予乙氧甲叉甲烷的雄性大鼠中牛乳铁蛋白对异常隐窝灶起始和早期发展的抑制作用及自然杀伤活性增强，《癌症通讯》121 (1997) 211 - 216），本研究在ApcMin小鼠（一种家族性腺瘤性息肉病和散发性结肠癌模型）中评估了其对自发性肠道息肉发展的化学预防作用。实验中，将54只6周龄小鼠随意给予2% bLF（15只小鼠）、0.2% bLF（15只小鼠）和AIN - 93G（24只小鼠）作为基础日粮，持续8周。在bLF处理的动物中，小肠息肉总数有总体减少的趋势，在给予2%剂量时空肠息肉有显著抑制（P < 0.05，为对照组的68%）。此外，因肠道内大量息肉导致的贫血和/或肠套叠所推测的身体生长抑制得到缓解。在肠道上皮未观察到毒性作用。尽管不如在大鼠实验中那么明显，但数据表明bLF可能是肠道息肉病的一种化学预防剂。
