Sekine K, Ushida Y, Kuhara T, Iigo M, Baba-Toriyama H, Moore M A, Murakoshi M, Satomi Y, Nishino H, Kakizoe T, Tsuda H
Chemotherapy Division, National Cancer Center Research Institute, Tokyo, Japan.
Cancer Lett. 1997 Dec 23;121(2):211-6. doi: 10.1016/s0304-3835(97)00358-3.
The influence of concomitant administration of bovine lactoferrin (bLF) on induction of aberrant crypt foci (ACF) by azoxymethane was investigated in male F344 rats. Two percent bLF and 3% Bifidobacterium longum (B. longum), as a positive control, significantly decreased the numbers of ACF as well as the total numbers of aberrant crypts reproducibly in three independent studies (2% bLF, P < 0.01; 3% B. longum, P < 0.05). Most importantly large size foci composed of four or more crypts were always significantly decreased by 2% bLF (P < 0.05). Additional investigation of the natural killer activity of spleen cells demonstrated enhancement by bLF (P < 0.01) and B. longum (P < 0.01) in line with the levels of influence on foci induction, indicating a possible role for elevated immune cytotoxicity in the observed inhibition.
在雄性F344大鼠中，研究了同时给予牛乳铁蛋白（bLF）对由氧化偶氮甲烷诱导的异常隐窝灶（ACF）的影响。作为阳性对照，2%的bLF和3%的长双歧杆菌（B. longum）在三项独立研究中均能显著降低ACF的数量以及异常隐窝的总数（2% bLF，P < 0.01；3% B. longum，P < 0.05）。最重要的是，由四个或更多隐窝组成的大尺寸病灶总是被2%的bLF显著减少（P < 0.05）。对脾细胞自然杀伤活性的进一步研究表明，bLF（P < 0.01）和B. longum（P < 0.01）可增强其活性，这与对病灶诱导的影响水平一致，表明免疫细胞毒性升高在观察到的抑制作用中可能发挥作用。
