Peterson B T, Miller E J, Morris D
Department of Cell Biology, University of Texas Health Center at Tyler 75710, USA.
Exp Lung Res. 1999 Jan-Feb;25(1):41-54. doi: 10.1080/019021499270411.
Single-cycle lavages performed on the lungs of rabbits 7 hours after they received intravenous staphylococcal enterotoxin-A (SEA) showed that most of the neutrophils were found in the second of 5 fractions of fluid removed from the lungs. To determine whether this finding reflected an accumulation of neutrophils in the airways versus the alveolar regions of the lungs, we counted the neutrophils in histologic sections (20 microns) and estimated the concentration of neutrophils in the epithelial lining fluid of the airways versus the alveoli. Comparisons of the morphometric measurements and the lavage data showed a strong correlation between the 2 methods (r = .90, P < .001), but the lavage technique underestimated the morphometric value by a factor of 7. The histologic examination confirmed that the concentration of neutrophils in the airways was 3-4 times higher than in the alveoli. There were no differences between the alveoli and the airways with respect to the concentration of endogenous proteins and the chemoattractant, interleukin (IL)-8. Lavages performed in additional rabbits 4 and 18 hours after injection of SEA showed that neutrophils most likely entered the alveolar region in response to SEA and migrated into the airways. The single-cycle technique provides a reliable measure of the relative concentration of neutrophils in the airways versus the alveoli.
在兔子静脉注射葡萄球菌肠毒素A（SEA）7小时后对其肺部进行单周期灌洗，结果显示，在从肺部吸出的5份液体中的第2份中发现了大多数中性粒细胞。为了确定这一发现是否反映了中性粒细胞在气道与肺的肺泡区域的积聚，我们在组织学切片（20微米）中对中性粒细胞进行计数，并估计气道与肺泡的上皮衬液中中性粒细胞的浓度。形态学测量结果与灌洗数据的比较显示，这两种方法之间存在很强的相关性（r = 0.90，P < 0.001），但灌洗技术将形态学值低估了7倍。组织学检查证实，气道中的中性粒细胞浓度比肺泡中的高3至4倍。在内源性蛋白质和趋化因子白细胞介素（IL）-8的浓度方面，肺泡和气道之间没有差异。在注射SEA后4小时和18小时对其他兔子进行的灌洗显示，中性粒细胞很可能因SEA而进入肺泡区域并迁移到气道中。单周期技术为测量气道与肺泡中中性粒细胞的相对浓度提供了一种可靠的方法。
