Martens-Lobenhoffer J, Neumann H J
Institute of Clinical Pharmacology, Otto-von-Guericke-University Magdeburg, Germany.
J Chromatogr B Biomed Sci Appl. 1999 Jan 8;721(1):135-40. doi: 10.1016/s0378-4347(98)00481-2.
The determination of the metabolites of histamine, 1-methylhistamine (MHIS) and 1-methylimidazoleacetic acid (MIIA), in human urine is a useful tool for the diagnosis of mastocytosis. MHIS was extracted under basic conditions with chloroform and derivatized with trifluoroacetic acid anhydride, MIIA was derivatized with pentafluorobenzylbromide prior to extraction under basic conditions and the derivative was extracted with chloroform. The samples were assayed by gas chromatography-mass spectrometry. Normal concentrations of MHIS (2.01 micromol l(-1)) and MIIA (21.3 micromol l(-1)) in healthy volunteers' urine samples are clearly detectable with coefficients of variation of 7.0 and 8.9%. Pathological concentrations of 10.1 and 113 micromol l(-1) for MHIS and MIIA, respectively, are quantifiable with coefficients of variation of 6.6 and 4.8%.
测定人尿中组胺代谢产物1 - 甲基组胺（MHIS）和1 - 甲基咪唑乙酸（MIIA）是诊断肥大细胞增多症的一项有用工具。在碱性条件下用氯仿提取MHIS，并用三氟乙酸酐进行衍生化；在碱性条件下提取MIIA之前，先用五氟苄基溴对其进行衍生化，然后用氯仿提取该衍生物。通过气相色谱 - 质谱联用仪对样品进行检测。健康志愿者尿液样本中MHIS的正常浓度为2.01微摩尔/升，MIIA的正常浓度为21.3微摩尔/升，变异系数分别为7.0%和8.9%，均可清晰检测到。MHIS和MIIA的病理浓度分别为10.1微摩尔/升和113微摩尔/升，变异系数分别为6.6%和4.8%，均可进行定量分析。
