Jones J G, Adams R A, Cook A M, Evans S A
School of Molecular and Medical Biosciences, University of Wales, Cardiff.
Br J Haematol. 1999 Jan;104(1):100-7. doi: 10.1046/j.1365-2141.1999.01135.x.
Various techniques have been used to assess the flow properties of blood and blood cells in a range of clinical situations. Filtration through microfilters offers a single technique for measuring the flow properties of all cellular components of blood in one experiment but depends on an assumed ability to recognize cells that make up <10% of leucocytes. The remaining leucocytes, labelled fast leucocytes, were previously presumed to be lymphocytes and granulocytes. This study confirmed the identities of these fast leucocytes as those of lymphocytes and granulocytes in undiluted blood. The transit time for lymphocytes (1.2 s) and granulocytes (1.6 s) is close to that recorded for fast leucocytes (1.7 s). The resistance of each type of blood cell to flow through 5 microm filters was defined in this study as the product of the concentration of that cell in blood and its transit time through a pore in the filter. The total resistance to flow of healthy blood through the filter is 4.46 x 10(7) s/ml and is attributed to plasma (2.7%), red cells (25.9%), fast leucocytes (25.3%) and slow leucocytes (46.1%). In a cohort of 21 men with peripheral arterial disease the total resistance was increased to 7.82 x 10(7) s/ml and attributed to plasma (1.5%), red cells (14.5%), fast leucocytes (21.0%) and slow leucocytes (63.00%). This analysis therefore provides a single test for assessing the flow properties of all the cellular components of blood and plasma.
在一系列临床情况下，人们运用了各种技术来评估血液及血细胞的流动特性。通过微滤器进行过滤提供了一种在一次实验中测量血液所有细胞成分流动特性的单一技术，但这取决于识别占白细胞不到10%的细胞的假定能力。其余的白细胞，即标记为快速白细胞的，以前被认为是淋巴细胞和粒细胞。本研究证实了这些快速白细胞在未稀释血液中就是淋巴细胞和粒细胞。淋巴细胞（1.2秒）和粒细胞（1.6秒）的通过时间与快速白细胞记录的通过时间（1.7秒）相近。在本研究中，每种血细胞通过5微米滤器的流动阻力被定义为该细胞在血液中的浓度与其通过滤器孔隙的通过时间的乘积。健康血液通过滤器的总流动阻力为4.46×10⁷秒/毫升，这归因于血浆（2.7%）、红细胞（25.9%）、快速白细胞（25.3%）和慢速白细胞（46.1%）。在一组21名患有外周动脉疾病的男性中，总阻力增加到7.82×10⁷秒/毫升，归因于血浆（1.5%）、红细胞（14.5%）、快速白细胞（21.0%）和慢速白细胞（63.00%）。因此，这种分析提供了一种评估血液和血浆所有细胞成分流动特性的单一测试。
