Penny R, Goldstein I P, Frasier S D
J Clin Endocrinol Metab. 1977 Apr;44(4):780-3. doi: 10.1210/jcem-44-4-780.
FSH and LH in acetone precipitates of timed overnight urine collections were measured by radioimmunoassay. FSH and LH urinary excretion extrapolated to 24 h was determined in 140 normal girls 3-16 years. Timed overnight urines were collected throughout a menstrual cycle from 3 normal adult women. FSH and LH progressively increased with age from 2.0 +/- 0.5 (FSH) and 2.0 +/- 0.4 (LH) IU/24 h for the 3--4 yr age group to 8.1 +/- 1.8 (FSH) and 11.5 +/- 3.8 (LH) IU/24 h for the 15--16 yr age group. Mean FSH and LH excretion for succeeding age groups (3--4, 5--6, 7--8, 9--10, 11--12, 13--14 and 15--16 years) increased significantly for the 5--6, 9--10 and 11--12 year age groups. Mean LH but not FSH excretion increased significantly for the 13--14 year age group. FSH and LH excretion increased progressively with stage of sexual development. In the 3 girls with established menstrual cycles. FSH (43.6 +/- 5.0 IU/24 h) and LH (113.7 +/- 7.8 IU/24 h) peaks occurred on the same day. Luteal phase excretion of FSH and LH was significantly lower than follicular phase excretion. Timed overnight urine collection allows integration of several hours of FSH and LH excretion.
采用放射免疫分析法测定定时留取的过夜尿丙酮沉淀物中的促卵泡激素（FSH）和促黄体生成素（LH）。测定了140名3至16岁正常女孩24小时的FSH和LH尿排泄量。从3名成年正常女性整个月经周期中定时留取过夜尿。FSH和LH排泄量随年龄增长而逐渐增加，3至4岁年龄组为2.0±0.5（FSH）和2.0±0.4（LH）IU/24小时，15至16岁年龄组为8.1±1.8（FSH）和11.5±3.8（LH）IU/24小时。后续年龄组（3至4岁、5至6岁、7至8岁、9至10岁、11至12岁、13至14岁和15至16岁）中，5至6岁、9至10岁和11至12岁年龄组的FSH和LH平均排泄量显著增加。13至14岁年龄组LH平均排泄量显著增加，而FSH平均排泄量未显著增加。FSH和LH排泄量随性发育阶段逐渐增加。在3名已建立月经周期的女孩中，FSH（43.6±5.0 IU/24小时）和LH（113.7±7.8 IU/24小时）峰值出现在同一天。FSH和LH的黄体期排泄量显著低于卵泡期排泄量。定时留取过夜尿可综合数小时的FSH和LH排泄量。
