Duchaine C, Mériaux A, Brochu G, Cormier Y
Unité de Recherche, l'Hôpital Laval, Quebec, Canada.
Am Ind Hyg Assoc J. 1999 Jan-Feb;60(1):89-95. doi: 10.1080/00028899908984426.
Pediococcus pentosaceus is a lactic-acid producing bacterium inoculated in hay to prevent hay deterioration. This study sought to verify the effect of this treatment on the barn microenvironment. Air samples were obtained from 19 barns using bacterial hay treatment and from 18 control barns with six-stage Andersen samplers and all-glass impingers. Appropriate culture media were used for the recovery and identification of microorganisms. Endotoxins were measured with chromogenic Limulus amoebocyte lysate assay. Median values (respectively for treated and untreated hay barns) were: 5.28 x 10(5) and 3.84 x 10(5) colony-forming units (CFU)/m3 for total bacteria; 3.18 x 10(6) and 4.5 x 10(6) CFU/m3 for molds; 1.36 x 10(3) and 1.74 x 10(3) endotoxin units/m3 for endotoxin levels; and 1.03 x 10(3) and 3.00 x 10(3) CFU/m3 for Saccharopolyspora rectivirgula. No viable P. pentosaceus were recovered. The presence of S. rectivirgula, the causative agent for farmer's lung, was not influenced by the hay treatment. Since no significant difference was observed in any of the airborne contaminants, this type of hay treatment probably does not protect farmers from the respiratory effect of ambient microbial contaminants.
戊糖片球菌是一种接种于干草中以防止干草变质的产乳酸细菌。本研究旨在验证这种处理对畜舍微环境的影响。使用细菌干草处理法从19个畜舍采集空气样本，并使用六级安德森采样器和全玻璃冲击式采样器从18个对照畜舍采集空气样本。使用适当的培养基来回收和鉴定微生物。采用显色鲎试剂法测定内毒素。（处理过的和未处理的干草畜舍的）中位数分别为：总细菌数5.28×10⁵和3.84×10⁵菌落形成单位（CFU）/立方米；霉菌3.18×10⁶和4.5×10⁶CFU/立方米；内毒素水平1.36×10³和1.74×10³内毒素单位/立方米；以及直丝糖多孢菌1.03×10³和3.00×10³CFU/立方米。未回收活的戊糖片球菌。农民肺的病原体直丝糖多孢菌的存在不受干草处理的影响。由于在任何空气传播污染物中均未观察到显著差异，这种干草处理方式可能无法保护农民免受环境微生物污染物的呼吸影响。
