Pena A L, Lino C M, Silveira I N
University of Coimbra, Faculty of Pharmacy, Laboratory of Bromatology, Portugal.
J AOAC Int. 1999 Jan-Feb;82(1):55-60.
A multiresidue method for isolation and liquid chromatographic determination of oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) in milk is presented. The sensitivity of the method is adequate to meet the needs of regulatory agencies. The European Community established 100 micrograms/kg as the maximum residue limit (MRL) in milk for TC, CTC, and OTC. Recoveries exceeded 80% for all tetracyclines at all levels, with good precision. Correlation coefficients of standards curves for individual tetracyclines isolated from fortified samples ranged from 0.991 for CTC to 0.998 for OTC. Other antibiotics that might interfere with analysis did not interfere with elution times of OTC, TC, and CTC. The procedure is rapid, precise, and quantitative and requires minimal preparation and minimal use of organic solvents. It can be applied to routine surveillance programs. We can prepare 10 samples for analysis in about 1.45 h.
本文介绍了一种用于分离和液相色谱测定牛奶中土霉素（OTC）、四环素（TC）和金霉素（CTC）的多残留方法。该方法的灵敏度足以满足监管机构的需求。欧盟规定牛奶中TC、CTC和OTC的最大残留限量（MRL）为100微克/千克。所有四环素在各个添加水平下的回收率均超过80%，精密度良好。从加标样品中分离出的各四环素标准曲线的相关系数范围为：CTC为0.991，OTC为0.998。其他可能干扰分析的抗生素不会干扰OTC、TC和CTC的洗脱时间。该方法快速、精确且定量，所需样品制备和有机溶剂用量极少。它可应用于常规监测项目。我们大约能在1.45小时内制备10个用于分析的样品。
