Zhu J, Snow D D, Cassada D A, Monson S J, Spalding R F
Water Center, Institute of Agriculture and Natural Resources, University of Nebraska-Lincoln, 68583-0844, USA.
J Chromatogr A. 2001 Sep 14;928(2):177-86. doi: 10.1016/s0021-9673(01)01139-6.
A method using liquid chromatography-tandem mass spectrometry has been developed for determination of trace levels of tetracycline antibiotics in ground water and confined animal feeding operation waste water. Oxytetracycline (OTC), tetracycline (TC), and chlortetracycline (CTC) were extracted from water samples using both polymeric and C18 extraction cartridges. The addition of a buffer containing potassium phosphate and citric acid improved tetracycline recoveries in lagoon water. Method detection limits determined in reagent water fortified with 1 microg l(-1) OTC, TC, and CTC were 0.21, 0.20, and 0.28 microg l(-1). Method detection limits in lagoon water samples fortified at 20 microg l(-1) for OTC, TC, and CTC were 3.6, 3.1, and 3.8 microg l(-1). Variability in recovery from laboratory fortified blanks ranged from 86 to 110% during routine analysis.
已开发出一种使用液相色谱 - 串联质谱法测定地下水和集约化养殖场废水中痕量四环素类抗生素的方法。使用聚合物萃取柱和C18萃取柱从水样中萃取土霉素（OTC）、四环素（TC）和金霉素（CTC）。添加含磷酸钾和柠檬酸的缓冲液可提高泻湖水中四环素的回收率。在添加了1 μg l⁻¹ OTC、TC和CTC的试剂水中测定的方法检出限分别为0.21、0.20和0.28 μg l⁻¹。在添加浓度为20 μg l⁻¹ OTC、TC和CTC的泻湖水样中，方法检出限分别为3.6、3.1和3.8 μg l⁻¹。在常规分析期间，实验室加标空白样品的回收率变化范围为86%至110%。
