Warner C, Fekadu M, Whitfield S, Shaddock J
Rabies Section, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
J Virol Methods. 1999 Jan;77(1):69-74. doi: 10.1016/s0166-0934(98)00136-0.
Seventy anti-rabies virus monoclonal antibodies (Mabs) were tested for reactivity with rabies and rabies-related viruses in formalin-fixed (FF) tissues. Forty-three of the Mabs were directed against the glycoprotein and 27 were directed against the nucleocapsid as determined by enzyme immunoassays and neutralization tests. Twenty of the anti-glycoprotein Mabs and one of the anti-nucleocapsid Mabs reacted with the rabies challenge virus strain (CVS) in FF tissue. These 21 Mabs were screened against other lyssaviruses in FF tissues: five rabies virus strains (coyote, skunk, raccoon, red bat, and silver-haired bat), and four rabies-related viruses (Australian bat lyssavirus, Duvenhage virus, Lagos bat virus, and Mokola virus). One of the anti-glycoprotein Mabs was reactive with all the virus strains screened. Another of the anti-glycoprotein Mabs reacted with all of the rabies virus strains tested, but not with any of the rabies-related virus strains tested. The remaining Mabs had reactivity patterns that could be useful for characterizing lyssaviruses in FF tissues.
对70种抗狂犬病病毒单克隆抗体（Mabs）进行了检测，以确定其与福尔马林固定（FF）组织中的狂犬病病毒和狂犬病相关病毒的反应性。通过酶免疫测定和中和试验确定，其中43种Mabs针对糖蛋白，27种针对核衣壳。20种抗糖蛋白Mabs和1种抗核衣壳Mabs在FF组织中与狂犬病攻击病毒株（CVS）发生反应。对这21种Mabs在FF组织中针对其他狂犬病病毒进行了筛选：5种狂犬病病毒株（郊狼、臭鼬、浣熊、红蝙蝠和银毛蝙蝠），以及4种狂犬病相关病毒（澳大利亚蝙蝠狂犬病病毒、杜文海格病毒、拉各斯蝙蝠病毒和莫科拉病毒）。其中1种抗糖蛋白Mabs与所有筛选的病毒株发生反应。另一种抗糖蛋白Mabs与所有测试的狂犬病病毒株发生反应，但与任何测试的狂犬病相关病毒株均无反应。其余的Mabs具有可用于在FF组织中鉴定狂犬病病毒的反应模式。
