一种采用聚乙二醇来测定特异性抗体对干扰素中和作用的生物测定法。

A bioassay employing polyethylene glycol for measuring neutralization of interferon by specific antibodies.

作者信息

Inglot A D, Chudzio T

出版信息

J Immunol Methods. 1976;13(1):63-9. doi: 10.1016/0022-1759(76)90187-3.

Abstract

A bioassay employing polyethylene glycol (PEG) for measuring the level of interferon-neutralizing antibodies is described. The method is based on incubating a concentrated preparation of interferon with an appropriately diluted anti-interferon serum or globulin for 1 h at 37 degrees C, precipitating the antigen-antibody complexes as well as some free serum protein with sterile PEG at a final concentration of 10% for 18 h at 4 degrees C, separating the sediment by centrifugation, and titrating the residual interferon in the supernate. It is not necessary to remove PEG from the samples since it is not toxic for tissue culture at a final concentration of less than 1%.

摘要

本文描述了一种使用聚乙二醇（PEG）来测量干扰素中和抗体水平的生物测定法。该方法基于将浓缩的干扰素制剂与适当稀释的抗干扰素血清或球蛋白在37℃下孵育1小时，然后用终浓度为10%的无菌PEG在4℃下沉淀抗原-抗体复合物以及一些游离血清蛋白18小时，通过离心分离沉淀物，并滴定上清液中残留的干扰素。由于最终浓度低于1%的PEG对组织培养无毒，因此无需从样品中去除PEG。