Vanniasinkam T, Lanser J A, Barton M D
School of Pharmacy and Medical Sciences, University of South Australia, Australia.
Lett Appl Microbiol. 1999 Jan;28(1):52-6. doi: 10.1046/j.1365-2672.1999.00474.x.
The suitability of PCR (based on the amplification of the 16S rRNA gene) for use as a diagnostic test for the detection of Campylobacter spp. in human faecal specimens was assessed. A total of 493 faecal specimens from patients with symptoms of enteritis were tested for the presence of campylobacters using PCR. Results were compared with those obtained from the analyses of the same specimens by culture techniques, using chi 2 square with Fisher's exact test. PCR was found to detect significantly more positive specimens than culture (chi 2 = 200.086; P < 0.0001). The sensitivity and specificity of PCR when compared with the culture technique were found to be 91 and 97%, respectively. It is proposed that the PCR is a reliable and sensitive method which may be used as a routine diagnostic technique for the detection of campylobacters in clinical specimens.
评估了聚合酶链反应（基于16S rRNA基因扩增）作为检测人类粪便标本中弯曲杆菌属诊断试验的适用性。使用聚合酶链反应对493份来自有肠炎症状患者的粪便标本进行弯曲杆菌检测。通过培养技术对相同标本进行分析，并使用卡方检验和费舍尔精确检验将结果进行比较。发现聚合酶链反应检测出的阳性标本显著多于培养法（卡方 = 200.086；P < 0.0001）。与培养技术相比，聚合酶链反应的敏感性和特异性分别为91%和97%。建议聚合酶链反应是一种可靠且灵敏的方法，可作为临床标本中弯曲杆菌检测的常规诊断技术。
