用于检测和鉴定弯曲杆菌属菌种的聚合酶链反应-酶联免疫吸附测定：应用于分离株和粪便样本
PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.
作者信息
Metherell L A, Logan J M, Stanley J
机构信息
Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom.
出版信息
J Clin Microbiol. 1999 Feb;37(2):433-5. doi: 10.1128/JCM.37.2.433-435.1999.
Abstract
We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.
摘要
我们报告了一种聚合酶链反应-酶联免疫吸附测定法，该方法通过在微量滴定板中用种特异性探针捕获单链16S rRNA基因扩增子进行杂交来鉴定弯曲杆菌属菌种。已证实该方法对参考菌株和现场菌株均具有特异性，但空肠弯曲杆菌的模式菌株不典型。该测定法快速、信息丰富，并且可用于粪便提取的DNA。
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