• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于检测和鉴定弯曲杆菌属菌种的聚合酶链反应-酶联免疫吸附测定：应用于分离株和粪便样本

PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.

作者信息

Metherell L A, Logan J M, Stanley J

机构信息

Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, London NW9 5HT, United Kingdom.

出版信息

J Clin Microbiol. 1999 Feb;37(2):433-5. doi: 10.1128/JCM.37.2.433-435.1999.

DOI:10.1128/JCM.37.2.433-435.1999
PMID:9889235
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC84331/
Abstract

We report a PCR-enzyme-linked immunosorbent assay which identifies Campylobacter species by capture hybridization of a single-stranded 16S rRNA gene amplicon with species-specific probes in a microtiter plate format. Specificities were confirmed for both reference and field strains, but the type strain of Campylobacter coli was atypical. The assay was rapid, informative, and usable with stool-extracted DNA.

摘要

我们报告了一种聚合酶链反应-酶联免疫吸附测定法，该方法通过在微量滴定板中用种特异性探针捕获单链16S rRNA基因扩增子进行杂交来鉴定弯曲杆菌属菌种。已证实该方法对参考菌株和现场菌株均具有特异性，但空肠弯曲杆菌的模式菌株不典型。该测定法快速、信息丰富，并且可用于粪便提取的DNA。

相似文献

1
PCR-enzyme-linked immunosorbent assay for detection and identification of Campylobacter species: application to isolates and stool samples.用于检测和鉴定弯曲杆菌属菌种的聚合酶链反应-酶联免疫吸附测定：应用于分离株和粪便样本
J Clin Microbiol. 1999 Feb;37(2):433-5. doi: 10.1128/JCM.37.2.433-435.1999.
2
Rapid identification of Campylobacter spp. by melting peak analysis of biprobes in real-time PCR.通过实时PCR中双探针的熔解峰分析快速鉴定弯曲杆菌属。
J Clin Microbiol. 2001 Jun;39(6):2227-32. doi: 10.1128/JCM.39.6.2227-2232.2001.
3
Detection of Campylobacter in stool and determination of significance by culture, enzyme immunoassay, and PCR in developing countries.发展中国家粪便中弯曲杆菌的检测及通过培养、酶免疫测定和聚合酶链反应确定其意义
J Clin Microbiol. 2014 Apr;52(4):1074-80. doi: 10.1128/JCM.02935-13. Epub 2014 Jan 22.
4
Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.用于鉴定人粪便标本中弯曲杆菌属的聚合酶链反应/DNA探针比色膜分析法的评估
J Clin Microbiol. 2001 Nov;39(11):4163-5. doi: 10.1128/JCM.39.11.4163-4165.2001.
5
Detection and identification of Campylobacter spp. using the polymerase chain reaction.利用聚合酶链反应检测和鉴定弯曲杆菌属细菌。
Cell Mol Biol (Noisy-le-grand). 1995 Jul;41(5):625-38.
6
Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.用于检测粪便临床标本中弯曲杆菌属的培养方法及基于DNA探针的聚合酶链反应分析方法的评估。
J Clin Microbiol. 2003 Jul;41(7):2980-6. doi: 10.1128/JCM.41.7.2980-2986.2003.
7
Isolation of Campylobacter and identification by PCR.弯曲杆菌的分离及通过聚合酶链反应进行鉴定。
Methods Mol Biol. 2003;216:109-21. doi: 10.1385/1-59259-344-5:109.
8
Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods.弯曲杆菌属菌种的检测：基于培养法和聚合酶链反应法的比较
J Clin Pathol. 2002 Oct;55(10):749-53. doi: 10.1136/jcp.55.10.749.
9
Large-scale survey of Campylobacter species in human gastroenteritis by PCR and PCR-enzyme-linked immunosorbent assay.通过聚合酶链反应（PCR）和PCR-酶联免疫吸附测定法对人类肠胃炎中弯曲杆菌属进行大规模调查。
J Clin Microbiol. 1999 Dec;37(12):3860-4. doi: 10.1128/JCM.37.12.3860-3864.1999.
10
PCR for the detection of Campylobacter spp. in clinical specimens.用于检测临床标本中弯曲杆菌属的聚合酶链反应。
Lett Appl Microbiol. 1999 Jan;28(1):52-6. doi: 10.1046/j.1365-2672.1999.00474.x.

引用本文的文献

1
Updating the genomic taxonomy and epidemiology of Campylobacter hyointestinalis.更新弯曲杆菌属肠亚种的基因组分类学和流行病学。
Sci Rep. 2018 Feb 5;8(1):2393. doi: 10.1038/s41598-018-20889-x.
2
Development and evaluation of immunochromatographic assay for simple and rapid detection of Campylobacter jejuni and Campylobacter coli in human stool specimens.用于人粪便标本中空肠弯曲菌和结肠弯曲菌简易快速检测的免疫层析检测方法的开发与评估
J Clin Microbiol. 2008 Apr;46(4):1226-31. doi: 10.1128/JCM.02170-07. Epub 2008 Feb 6.
3
Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a multiplex PCR developed from the nucleotide sequence of the lipid A gene lpxA.通过基于脂质A基因lpxA核苷酸序列开发的多重PCR对空肠弯曲菌、结肠弯曲菌、拉氏弯曲菌和乌普萨拉弯曲菌进行鉴别。
J Clin Microbiol. 2004 Dec;42(12):5549-57. doi: 10.1128/JCM.42.12.5549-5557.2004.
4
Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.通过实时聚合酶链反应检测鸡粪便样本中的弯曲杆菌属。
J Clin Microbiol. 2004 Nov;42(11):5125-32. doi: 10.1128/JCM.42.11.5125-5132.2004.
5
Specificity and performance of PCR detection assays for microbial pathogens.用于微生物病原体的PCR检测方法的特异性和性能
Mol Biotechnol. 2004 Jan;26(1):61-80. doi: 10.1385/MB:26:1:61.
6
Microarray-based identification of thermophilic Campylobacter jejuni, C. coli, C. lari, and C. upsaliensis.基于微阵列技术对嗜热空肠弯曲菌、大肠弯曲菌、拉氏弯曲菌和乌普萨拉弯曲菌的鉴定
J Clin Microbiol. 2003 Sep;41(9):4071-80. doi: 10.1128/JCM.41.9.4071-4080.2003.
7
Evaluation of culture methods and a DNA probe-based PCR assay for detection of Campylobacter species in clinical specimens of feces.用于检测粪便临床标本中弯曲杆菌属的培养方法及基于DNA探针的聚合酶链反应分析方法的评估。
J Clin Microbiol. 2003 Jul;41(7):2980-6. doi: 10.1128/JCM.41.7.2980-2986.2003.
8
Detection of campylobacter species: a comparison of culture and polymerase chain reaction based methods.弯曲杆菌属菌种的检测：基于培养法和聚合酶链反应法的比较
J Clin Pathol. 2002 Oct;55(10):749-53. doi: 10.1136/jcp.55.10.749.
9
Evaluation of a PCR/DNA probe colorimetric membrane assay for identification of Campylobacter spp. in human stool specimens.用于鉴定人粪便标本中弯曲杆菌属的聚合酶链反应/DNA探针比色膜分析法的评估
J Clin Microbiol. 2001 Nov;39(11):4163-5. doi: 10.1128/JCM.39.11.4163-4165.2001.
10
Basis of the superiority of cefoperazone amphotericin teicoplanin for isolating Campylobacter upsaliensis from stools.头孢哌酮、两性霉素、替考拉宁从粪便中分离出犬弯曲杆菌的优势基础。
J Clin Microbiol. 2001 Jul;39(7):2713-6. doi: 10.1128/JCM.39.7.2713-2716.2001.

本文引用的文献

1
Polymerase chain reaction detection and speciation of Campylobacter upsaliensis and C. helveticus in human faeces and comparison with culture techniques.人粪便中乌普萨拉弯曲杆菌和瑞士弯曲杆菌的聚合酶链反应检测、鉴定及与培养技术的比较
J Appl Microbiol. 1997 Sep;83(3):375-80. doi: 10.1046/j.1365-2672.1997.00240.x.
2
PCR detection, identification to species level, and fingerprinting of Campylobacter jejuni and Campylobacter coli direct from diarrheic samples.直接从腹泻样本中对空肠弯曲菌和结肠弯曲菌进行PCR检测、种水平鉴定及指纹图谱分析。
J Clin Microbiol. 1997 Oct;35(10):2568-72. doi: 10.1128/jcm.35.10.2568-2572.1997.
3
Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animals.通过聚合酶链反应（PCR）快速鉴定弯曲杆菌属以及对人和动物具有致病性的五种弯曲杆菌。
Res Microbiol. 1996 Nov-Dec;147(9):707-18. doi: 10.1016/s0923-2508(97)85118-2.
4
PCR-enzyme-linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria.聚合酶链反应-酶联免疫吸附测定法与部分核糖体RNA基因测序：鉴定分枝杆菌的合理方法。
J Clin Microbiol. 1997 Sep;35(9):2375-80. doi: 10.1128/jcm.35.9.2375-2380.1997.
5
Intraspecific variation in small-subunit rRNA sequences in GenBank: why single sequences may not adequately represent prokaryotic taxa.GenBank中小亚基rRNA序列的种内变异：为何单条序列可能无法充分代表原核生物分类群。
Int J Syst Bacteriol. 1995 Jul;45(3):595-9. doi: 10.1099/00207713-45-3-595.
6
Chemotaxonomic analyses of Bacteroides gracilis and Bacteroides ureolyticus and reclassification of B. gracilis as Campylobacter gracilis comb. nov.纤细拟杆菌和解脲拟杆菌的化学分类分析以及纤细拟杆菌重新分类为纤细弯曲菌，新组合
Int J Syst Bacteriol. 1995 Jan;45(1):145-52. doi: 10.1099/00207713-45-1-145.
7
CLUSTAL V: improved software for multiple sequence alignment.CLUSTAL V：用于多序列比对的改进软件。
Comput Appl Biosci. 1992 Apr;8(2):189-91. doi: 10.1093/bioinformatics/8.2.189.