John N M, Zea M E, Kawano T, Omata Y, Saito A, Toyoda Y, Mikami T
Department of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Japan.
Vet Parasitol. 1999 Feb 25;81(2):99-105. doi: 10.1016/s0304-4017(98)00239-8.
The carbohydrates present on Eimeria stiedai sporozoites and their functional role in the process of invasion of host cells were examined. Lectin-binding sites on the surface of sporozoites were detected by means of peroxidase-conjugated lectins. Sporozoites showed specific binding with UEA-I and PNA lectins, which bind L-fucose and D-galactose, respectively. Exposure of sporozoites to 100 microg/ml UEA-I significantly reduced their ability to invade primary rabbit liver biliary epithelial cells, but similar treatment with PNA had no such effect. Pre-incubation of these cells in Dulbecco's minimum essential medium containing 10% fetal bovine serum and 1% L-fucose suppressed the invasion activity of the sporozoites, but pre-incubation of the sporozoites in the same medium without L-fucose had no effect on cell penetration. D-galactose added to the medium had no effect on the invasion activity of sporozoites. These results indicate that L-fucose residues on E. stiedai sporozoites and L-fucose-binding sites on host cells both are associated with the recognition and/or invasion process.
研究了斯氏艾美耳球虫子孢子表面存在的碳水化合物及其在宿主细胞入侵过程中的功能作用。通过过氧化物酶偶联凝集素检测子孢子表面的凝集素结合位点。子孢子与分别结合L-岩藻糖和D-半乳糖的UEA-I和PNA凝集素表现出特异性结合。将子孢子暴露于100μg/ml UEA-I中显著降低了它们侵入原代兔肝胆管上皮细胞的能力，但用PNA进行类似处理则没有这种效果。在含有10%胎牛血清和1% L-岩藻糖的杜尔贝科改良 Eagle 培养基中预孵育这些细胞可抑制子孢子的入侵活性，但在不含L-岩藻糖的相同培养基中预孵育子孢子对细胞穿透没有影响。向培养基中添加D-半乳糖对子孢子的入侵活性没有影响。这些结果表明，斯氏艾美耳球虫子孢子上的L-岩藻糖残基和宿主细胞上的L-岩藻糖结合位点均与识别和/或入侵过程有关。
