血清因子：对慢性淋巴细胞白血病淋巴细胞的植物血凝素诱导的母细胞形成的抑制作用。

Serum factor: the inhibition of phytohemagglutinin-induced blastogenesis of chronic lymphocytic leukemia lymphocytes.

作者信息

Monahan T M, Fritz R R, Abell C W

出版信息

J Natl Cancer Inst. 1976 Jul;57(1):217-20. doi: 10.1093/jnci/57.1.217.

DOI:10.1093/jnci/57.1.217

Abstract

A serum factor (SF), isolated from acidified sera of normal donors, inhibited phytohemagglutinin (PHA) stimulation of lymphocytes isolated from patients with chronic lymphocytic leukemia (CLL),but had no effect on those from healthy donors. An SF isolated by an identical procedure from the sera of patients with CLL had essentially no inhibitory effect on DNA synthesis in normal or leukemic lymphocytes. The SF was isolated from sera or plasma by ammonium sulfate precipitation, acidification to pH 1.5, and chromatography on DEAE-cellulose. The SF was heat labile and sensitive to digestion by trypsin. Maximum inhibition was obtained when the factor was added within 24 hours after the cells were stimulated with PHA.

摘要

从正常供体的酸化血清中分离出的一种血清因子（SF），可抑制从慢性淋巴细胞白血病（CLL）患者分离出的淋巴细胞对植物血凝素（PHA）的刺激反应，但对健康供体的淋巴细胞无作用。通过相同程序从CLL患者血清中分离出的SF对正常或白血病淋巴细胞的DNA合成基本没有抑制作用。该SF通过硫酸铵沉淀、酸化至pH 1.5以及在DEAE-纤维素上进行层析从血清或血浆中分离得到。该SF对热不稳定，且对胰蛋白酶消化敏感。在用PHA刺激细胞后24小时内加入该因子可获得最大抑制效果。