Goldin B R, Gorbach S L
J Natl Cancer Inst. 1976 Aug;57(2):371-5. doi: 10.1093/jnci/57.2.371.
We studied the effect of diet on the activities of four enzymes found in the intestinal flora of the male F344 rat. Animals initially fed a diet with high vegetable and grain content were shifted to a diet consisting predominantly of beef. While eating the meat diet, the rats had significantly higher levels of nitroreductase, azoreductase, and beta-glucuronidase in their fecal flora when compared to levels measured during grain feeding. However, beta-glucosidase activity was significantly lower during meat feeding, which probably reflected the lack of beta-glucosidic linkages in this diet. These findings suggested that a high-beef diet, similar in composition to that consumed by humans with a relatively high risk of colon cancer, is associated with elevated levels of specific enzymes in the colon microflora. These enzymes have been implicated in the conversion of procarcinogens into carcinogens.
我们研究了饮食对雄性F344大鼠肠道菌群中四种酶活性的影响。最初喂食高蔬菜和谷物含量饮食的动物被换成主要由牛肉组成的饮食。与喂食谷物期间测得的水平相比，大鼠在食用肉类饮食时，其粪便菌群中的硝基还原酶、偶氮还原酶和β-葡萄糖醛酸酶水平显著更高。然而，在食用肉类期间，β-葡萄糖苷酶活性显著降低，这可能反映了这种饮食中缺乏β-糖苷键。这些发现表明，一种高牛肉饮食，其成分与患结肠癌风险相对较高的人类所食用的饮食相似，与结肠微生物群中特定酶水平的升高有关。这些酶与将前致癌物转化为致癌物有关。
