Campana M A, Panzeri A M, Moreno V J, Dulout F N
Lab. Genética, Departamento de Biología, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Funes 3250, 3er piso, (7600), Mar del Plata, Argentina.
Mutat Res. 1999 Jan 13;438(2):155-61. doi: 10.1016/s1383-5718(98)00167-3.
In order to develop experimental models able to detect genotoxic effects of pollutants in aquatic organisms, the genotoxicity of the pyrethroid lambda-cyhalothrin was studied using the micronucleus test in erythrocytes of Cheirodon interruptus interruptus. The frequency of micronuclei was examined in blood smears obtained from fishes exposed in vivo to three different concentrations (0.05; 0. 01; 0.001 ug/l) of the compound and sacrificed at nine sampling times (24, 48, 72, 96 h and 8, 12, 15, 19 and 23 days). As a positive control fishes were exposed to 5 mg/l of cyclophosphamide. Results obtained demonstrated the genotoxic effects of the pyrethroid in the experimental model employed. The variation in the micronuclei frequencies in the different sampling times could be related to the blood cell kinetics and the erythrocyte replacement. The results could be considered as a validation of the MN test in fishes for the assessment of genotoxic pollutants.
为了建立能够检测污染物对水生生物遗传毒性效应的实验模型，利用中断丽脂鲤红细胞微核试验研究了拟除虫菊酯高效氯氟氰菊酯的遗传毒性。在体内暴露于该化合物三种不同浓度（0.05；0.01；0.001微克/升）并在九个采样时间点（24、48、72、96小时以及8、12、15、19和23天）处死的鱼的血涂片上检测微核频率。作为阳性对照，鱼暴露于5毫克/升的环磷酰胺。所获得的结果证明了在所采用的实验模型中拟除虫菊酯的遗传毒性效应。不同采样时间微核频率的变化可能与血细胞动力学和红细胞更替有关。这些结果可被视为鱼类微核试验用于评估遗传毒性污染物的一种验证。
