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[45孔VITEK药敏卡的快速性和质量研究]

[Study of rapidity and quality for the VITEK susceptibility cards with 45 wells].

作者信息

Miyamoto H, Handa T, Inoue C, Murase M

机构信息

The Clinical Laboratory, Ehime University Hospital, Shitsukawa, Shigenobu-cho, Onsen-gun, Ehime 791-0295, Japan.

出版信息

Rinsho Biseibutshu Jinsoku Shindan Kenkyukai Shi. 1998;9(2):55-8.

Abstract

VITEK 45 wells Card was evaluated. Recent clinical isolates of gram-positive cocci (107) and gram-negative bacilli (179) were antimicrobial susceptibility testing by VITEK system in comparison with MIC-2000 system. The antimicrobial susceptibility results were available within of 8 h for gram-positive cocci without a part of MRSA. Enterobac-teriaceae were available within of 8 h and Pseudomonas aeruginosa were from 8 to 11 h. The antimicrobial susceptibility data had disagreement of 60% with EM, 46% with CLDM for Enterococcus faecium, 50% with CMZ for Citrobacter freundii, and 71% with CTM for Morganella morganii, whereas it was satisfactory agreement of 97.5% for gram-positive cocci and 98.2% for gram-negative bacilli. In using this procedure it was possible to provide accurate and rapid results of anti-microbial susceptibility tests for all organisms.

摘要

对VITEK 45孔卡片进行了评估。采用VITEK系统对近期分离出的革兰氏阳性球菌(107株)和革兰氏阴性杆菌(179株)进行抗菌药物敏感性试验,并与MIC - 2000系统进行比较。对于不包括耐甲氧西林金黄色葡萄球菌(MRSA)的革兰氏阳性球菌,8小时内可获得抗菌药物敏感性结果。肠杆菌科细菌在8小时内可获得结果,铜绿假单胞菌在8至11小时内可获得结果。粪肠球菌的抗菌药物敏感性数据与纸片扩散法(EM)的不一致率为60%,与氯碳头孢(CLDM)的不一致率为46%,弗氏柠檬酸杆菌与头孢孟多(CMZ)的不一致率为50%,摩根摩根菌与头孢噻肟(CTM)的不一致率为71%,而革兰氏阳性球菌的一致性令人满意,为97.5%,革兰氏阴性杆菌的一致性为98.2%。使用该方法可以为所有微生物提供准确、快速的抗菌药物敏感性试验结果。

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