Seta Y, Toyono T, Takeda S, Toyoshima K
Department of Oral Anatomy II, Kyushu Dental College, Kitakyushu, Japan.
FEBS Lett. 1999 Feb 5;444(1):43-6. doi: 10.1016/s0014-5793(99)00023-x.
Mash1, a mammalian homologue of the Drosophila achaete-scute proneural gene complex, plays an essential role in differentiation of subsets of peripheral neurons. In this study, using RT-PCR and in situ RT-PCR, we investigated if Mash1 gene expression occurs in rat taste buds. Further, we examined dynamics of Mash1 expression in the process of degeneration and regeneration in denervated rat taste buds. In rat tongue epithelium, Mash1 gene expression is confined to circumvallate, foliate, and fungiform papilla epithelia that include taste buds. In taste buds, Mash1-expressing cells are round cells in the basal compartment. In contrast, the mature taste bud cells do not express the Mash1 gene. Denervation and regeneration experiments show that the expression of Mash1 requires gustatory innervation. We conclude that Mash1 is expressed in cells of the taste bud lineage, and that the expression of Mash1 in rat taste buds is dependent upon gustatory innervation.
Mash1是果蝇achaete-scute原神经基因复合体的哺乳动物同源物，在外周神经元亚群的分化中起重要作用。在本研究中，我们使用逆转录聚合酶链反应（RT-PCR）和原位RT-PCR来研究Mash1基因表达是否在大鼠味蕾中出现。此外，我们还检测了失神经支配的大鼠味蕾在退化和再生过程中Mash1表达的动态变化。在大鼠舌上皮中，Mash1基因表达局限于包含味蕾的轮廓乳头、叶状乳头和菌状乳头的上皮。在味蕾中，表达Mash1的细胞是基底部的圆形细胞。相比之下，成熟的味蕾细胞不表达Mash1基因。去神经支配和再生实验表明，Mash1的表达需要味觉神经支配。我们得出结论，Mash1在味蕾谱系的细胞中表达，并且大鼠味蕾中Mash1的表达依赖于味觉神经支配。
