基于模板转换效应和步移PCR的cDNA末端扩增
Amplification of cDNA ends based on template-switching effect and step-out PCR.
作者信息
Matz M, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A
机构信息
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, Moscow 117871, Russia.
出版信息
Nucleic Acids Res. 1999 Mar 15;27(6):1558-60. doi: 10.1093/nar/27.6.1558.
Abstract
A new method for amplifying cDNA ends is described which requires only first-strand cDNA synthesis and a single PCR to generate a correct product with very low or no background. The method can be successfully applied to total RNA as well as poly A+ RNA. The same first-strand cDNA can be used to amplify flanking sequences of any cDNA species present in the sample.
摘要
本文描述了一种用于扩增cDNA末端的新方法，该方法仅需进行第一链cDNA合成和一次PCR，即可产生背景极低或无背景的正确产物。该方法可成功应用于总RNA以及聚腺苷酸RNA。相同的第一链cDNA可用于扩增样品中存在的任何cDNA物种的侧翼序列。
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