荆门病毒组代表物 5' 和 3' 非翻译区的保守序列。

Conserved Sequences in the 5' and 3' Untranslated Regions of Jingmenvirus Group Representatives.

机构信息

Laboratory of Biology of Arboviruses, FSASI "Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of RAS" (Institute of Poliomyelitis), 108819 Moscow, Russia.

Department of Biology, Lomonosov Moscow State University, 119234 Moscow, Russia.

出版信息

Viruses. 2023 Apr 15;15(4):971. doi: 10.3390/v15040971.

DOI:10.3390/v15040971

PMID:37112951

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10141212/

Abstract

The Jingmenvirus group (JVG), with members such as Jingmen tick virus (JMTV), Alongshan virus (ALSV), Yanggou tick virus (YGTV), and Takachi virus (TAKV), is drawing attention due to evidence of it causing disease in humans and its unique genome architecture. In the current work, complete untranslated regions (UTRs) of four strains of ALSV and eight strains of YGTV were obtained. An analysis of these sequences, as well as JVG sequences from GenBank, uncovered several regions within viral UTRs that were highly conserved for all the segments and viruses. Bioinformatics predictions suggested that the UTRs of all the segments of YGTV, ALSV, and JMTV could form similar RNA structures. The most notable feature of these structures was a stable stem-loop with one (5' UTR) or two (3' UTR) AAGU tetraloops on the end of a hairpin.

摘要

荆门病毒组（JVG）包括荆门蜱病毒（JMTV）、鞍山病毒（ALSV）、羊沟蜱病毒（YGTV）和高知病毒（TAKV）等成员，由于有证据表明其在人类中引起疾病以及其独特的基因组结构而引起关注。在目前的工作中，获得了四种 ALSV 和八种 YGTV 毒株的完整非翻译区（UTR）。对这些序列以及来自 GenBank 的 JVG 序列的分析揭示了病毒 UTR 内的几个高度保守的区域，所有片段和病毒都具有这些区域。生物信息学预测表明，YGTV、ALSV 和 JMTV 的所有片段的 UTR 都可以形成类似的 RNA 结构。这些结构最显著的特征是在发夹的末端有一个稳定的茎环，其末端有一个（5'UTR）或两个（3'UTR）AAGU 四环。