Malashenko A M, Beskova T B
Genetika. 1988 Mar;24(3):468-73.
The sensitivity of spermatogonia of 101, TPS and CBA inbred mice to induction of chromosome damages by thiophosphamide (thioTEPA) was studied. With respect to sensitivity of bone marrow cells, these strains were ranked as follows: TPS greater than 101 greater than CBA. When used at the dose 2.25 mg/kg i/p thioTEPA induced chromosome damages in 11.4, 11.6 and 6.0% of spermatogonia of TPS, 101 and CBA mice, respectively. Thus, selection which increased sensitivity of bone marrow cells of TPS mice poorly affected germ cells. To obtain more correct estimations of genetically determined mouse strain sensitivity to chemical mutagens, the estimations should be based on frequencies of induced mutations in germ cells. The spectrum of mutations in spermatogonia differs from that in bone marrow cells: the frequencies of isochromatid breaks and symmetrical chromatid exchanges are higher in spermatogonia. This can be due to close situation of homologous chromosomes in interphase nuclei of spermatogonia.
研究了101、TPS和CBA近交系小鼠精原细胞对硫磷酰胺（噻替派）诱导染色体损伤的敏感性。就骨髓细胞敏感性而言，这些品系的排序如下：TPS＞101＞CBA。当腹腔注射剂量为2.25mg/kg时，硫磷酰胺分别在11.4%、11.6%和6.0%的TPS、101和CBA小鼠精原细胞中诱导染色体损伤。因此，提高TPS小鼠骨髓细胞敏感性的选择对生殖细胞影响较小。为了更准确地评估遗传决定的小鼠品系对化学诱变剂的敏感性，评估应基于生殖细胞中诱导突变的频率。精原细胞中的突变谱与骨髓细胞不同：等臂染色单体断裂和对称染色单体交换的频率在精原细胞中更高。这可能是由于精原细胞间期核中同源染色体的紧密状态所致。
