Fernandez S M, Berlin R D
Nature. 1976 Dec 2;264(5585):411-5. doi: 10.1038/264411a0.
The surface topography of concanavalin A (con A) bound to normal and transformed murine fibroblasts has been studied by a new technique involving fluorescence resonance energy transfer (RET), RET can provide a high resolution "map" of the distances separating con A-receptor complexes in single living cells. The distribution of con A is non-random in both normal and transformed cells, but sites are more closely approximated in the transformed. Approximation is induced by the con A but occurs at extremely slow rates indicating that the topography is not primarily determined by simple diffusion of complexes.
通过一种涉及荧光共振能量转移（RET）的新技术，研究了与正常和转化的小鼠成纤维细胞结合的伴刀豆球蛋白A（伴刀豆球蛋白A）的表面形貌。RET可以提供单个活细胞中伴刀豆球蛋白A - 受体复合物之间距离的高分辨率“图谱”。伴刀豆球蛋白A在正常细胞和转化细胞中的分布都是非随机的，但在转化细胞中位点更为接近。接近是由伴刀豆球蛋白A诱导的，但发生速率极慢，这表明形貌并非主要由复合物的简单扩散决定。
