Ohta N, Saito H, Kuzumaki T, Takahashi T, Ito M M, Saito T, Nakahara K, Hiroi M
Department of Obstetrics and Gynecology, Yamagata University School of Medicine, Yamagata City, Japan.
Mol Hum Reprod. 1999 Jan;5(1):22-8. doi: 10.1093/molehr/5.1.22.
CD44 is a polymorphic and polyfunctional transmembrane glycoprotein widely expressed in many types of cells. Here, the expression of this protein on human membrana granulosa was studied by two techniques. Using confocal laser scanning microscopy (CLSM) with the mouse monoclonal antibody to human CD44 (clone G44-26), cells immunoreactive for CD44 were observed in both cumulus and mural granulosa cell masses. On the other hand, using monoclonal antibody to human CD44v9, goat polyclonal antibody to human CD44v3-10 and the clone G44-26, no immunoreactivity for CD44v9 and/or CD44v3-10 was observed in either cell group by flow cytometry. In the flow cytometric analysis of 32 patients, the incidence of CD44 expression in cumulus cells (62.6+/-1.3%) was significantly higher than that in mural granulosa cells (38.5+/-3.2%) (P<0.0001). In the comparison of CD44 expression by flow cytometry according to the maturation of each cumulus-oocyte complex, the incidence of CD44 expression of cumulus cells was significantly higher in the mature group than in the immature group (P<0.05). In a flow cytometric analysis, patients with endometriosis showed a significantly lower incidence of CD44 expression in cumulus cells compared to the infertility of unknown origin group (P<0.05), and compared to both the male infertility group and the unknown origin group in mural granulosa cells (P<0.01). These findings suggest that the standard form of CD44 is expressed in human membrana granulosa with polarity and may play an important role in oocyte maturation.
CD44是一种多态性和多功能的跨膜糖蛋白，广泛表达于多种类型的细胞中。在此，通过两种技术研究了该蛋白在人颗粒膜上的表达。使用针对人CD44的小鼠单克隆抗体（克隆G44 - 26）的共聚焦激光扫描显微镜（CLSM），在卵丘和壁层颗粒细胞团中均观察到对CD44有免疫反应性的细胞。另一方面，使用针对人CD44v9的单克隆抗体、针对人CD44v3 - 10的山羊多克隆抗体以及克隆G44 - 26，通过流式细胞术在任一细胞组中均未观察到对CD44v9和/或CD44v3 - 10的免疫反应性。在对32例患者的流式细胞术分析中，卵丘细胞中CD44表达的发生率（62.6±1.3%）显著高于壁层颗粒细胞（38.5±3.2%）（P<0.0001）。根据每个卵丘 - 卵母细胞复合体的成熟度通过流式细胞术比较CD44表达时，成熟组卵丘细胞中CD44表达的发生率显著高于未成熟组（P<0.05）。在流式细胞术分析中，与不明原因不孕症组相比，子宫内膜异位症患者卵丘细胞中CD44表达的发生率显著降低（P<0.05），与男性不育组和不明原因组相比，壁层颗粒细胞中CD44表达的发生率也显著降低（P<0.01）。这些发现表明，CD44的标准形式在人颗粒膜上呈极性表达，可能在卵母细胞成熟中起重要作用。
