IVF Unit, Department of Obstetrics and Gynecology, Chaim Sheba Medical Centre, Tel-Hashomer, Affiliated to the Sackler School of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
J Assist Reprod Genet. 2011 Jun;28(6):509-15. doi: 10.1007/s10815-011-9594-9. Epub 2011 Jun 11.
Ovarian follicular development and ovulation in mammals is a complex and highly regulated process. Most advances in the understanding of the ovulatory process have come from animal models. However, translational research in humans is of crucial importance for improving fertility treatment and control.
IVM/IVF procedures allow us to obtain follicular fluid and granulosa cells (GC) from follicles in different developmental stages with and without hCG priming.
Using the cells and fluids obtained in IVM/IVF procedures allowed us to characterize human ovulatory gene expression during antral folliculogenesis and ovulation, examine gene expression in luteinized and non-luteinized GC in vivo and in vitro and to use cumulus GC genes as biomarkers for oocyte and embryo maturity and competence.
Biological material obtained during IVM/IVF procedures is an important tool to study the human ovulatory cascade and can serve to improve IVM techniques and fertility treatment and control.
哺乳动物的卵巢卵泡发育和排卵是一个复杂且高度调控的过程。大多数对排卵过程的理解都来自于动物模型。然而，对人类的转化研究对于改善生育治疗和控制至关重要。
IVM/IVF 程序允许我们从有和没有 hCG 引发的不同发育阶段的卵泡中获得卵泡液和颗粒细胞（GC）。
使用 IVM/IVF 程序中获得的细胞和液体，我们能够在窦前卵泡发生和排卵过程中表征人类排卵基因表达，研究体内和体外黄体化和非黄体化 GC 的基因表达，并使用卵丘 GC 基因作为卵母细胞和胚胎成熟和能力的生物标志物。
IVM/IVF 程序中获得的生物材料是研究人类排卵级联的重要工具，并可用于改进 IVM 技术和生育治疗和控制。
