使用不依赖引物的多核苷酸磷酸化酶进行特定寡核糖核苷酸的合成。
Specific oligoribonucleotide synthesis using primer-independent polynucleotide phosphorylase.
作者信息
Havier R G, Straus D B
出版信息
Prep Biochem. 1975;5(5-6):423-32. doi: 10.1080/00327487508061589.
PMID:175364
Abstract
Oligoribonucleotides of a predetermined base sequence beginning with adenylyl-3', 5'-adenosine at the 5' end have been synthesized in yields varying between 13% and 42%. The synthesis was carried out using primer-independent polynucleotide phosphorylase from E. coli in the presence of high concentrations of primer and of sodium chloride.
摘要
已合成了5'端以腺苷酰-3',5'-腺苷开头的具有预定碱基序列的寡核糖核苷酸，产率在13%至42%之间变化。该合成是在高浓度引物和氯化钠存在的情况下，使用来自大肠杆菌的不依赖引物的多核苷酸磷酸化酶进行的。
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