Ellis D M, Dean D H
J Virol. 1986 Apr;58(1):223-4. doi: 10.1128/JVI.58.1.223-224.1986.
Chromosomal DNAs of lysogens of phi 105 and phi 105 DI:1t were digested with restriction enzymes EcoRI and HpaI and were probed with nick-translated mature phi 105 DNA. Altered bacteriophage-specific bands in the lysogens were detected, indicating that the phage integrates into the host chromosome at a single site, probably via a Campbell-type circular intermediate. The phage attachment site is centrally located in the phage genome and lies between the phage immunity region and the nonessential deletable region of phi 105.
用限制性内切酶EcoRI和HpaI消化φ105和φ105 DI:1t溶原菌的染色体DNA，并用缺口平移法制备的成熟φ105 DNA进行探针杂交。在溶原菌中检测到噬菌体特异性条带的改变，表明噬菌体可能通过坎贝尔型环状中间体在单个位点整合到宿主染色体中。噬菌体附着位点位于噬菌体基因组的中心位置，在噬菌体免疫区域和φ105的非必需可缺失区域之间。
