Norlén L, Dahlström A, Sundin T, Svedmyr N
Scand J Urol Nephrol. 1976;10(3):177-84. doi: 10.1080/21681805.1976.11882070.
After long-term parasymphatetic denervation, detrusor muscle hypertrophy was found regardless of whether the hypogastric nerves had been sectioned simultaneously or not. After hypogastric denervation only, there was no difference in gross appearance from normal bladders. Adrenergic innervation was studied by means of a histochemical fluorescence technique. After hypogastric denervation, there was usually a slight increase in the detrusor innervation, and a clear decrease in the trigone and urethral adrenergic innervation. After parasympathetic denervation, the detrusor showed an increase in adrenergic innervation, while the innervation of the trigone and the urethra was unchanged. After simultaneous parasympathetic and hypogastric denervation, the adrenergic innervation of the detrusor was similar to that of the bladders subjected to parasympathetic denervation only. The adrenergic innervation of the trigone and urethra was similar to that found after hypogastric denervation only. Adrenergic receptor functions were studied by a method in which changes in the isometric tension of muscle strips were recorded following different pharmacological treatments. In the normal state, noradrenalin and phenylephrine caused contraction and isoprenaline relaxation of the trigone-urethra. In the detrusor, noradrenalin caused relaxation of strips contracted by carbacholine. Relaxed detrusor muscle strips were usually not contracted by noradenalin. In 2 out of 8 normal cats, however, a contraction was observed after very large doses of noradrenalin. After hypogastric denervation, the adrenoreceptor functions were consistent with those of the normal state. After parasympathetic denervation, the trigone-urethral receptor functions were unchanged compared with the normal receptor functions. In the detrusor, isoprenaline caused relaxation and noradrenalin and phenylephrine contraction. After simultaneous parasympathetic and hypogastric denervation, the results were consistent with those obtained after parasympathetic denervation only. Thus, the present study showed an increased adrenergic detrusor innervation and alpha-receptor activity after parasympathetic denervation regardless of whether the hypogastric nerves had been sectioned simultaneously or not. Although alpha-receptors might exist in the normal detrusor, beta-receptors dominate strongly. After parasympathetic denervation, there seems to be a change in the relation between alpha- and beta-receptors in favour of alpha-receptors.
长期副交感神经去神经支配后，无论下腹下神经是否同时切断，均发现逼尿肌肥大。仅行下腹下神经去神经支配后，膀胱外观与正常膀胱无差异。采用组织化学荧光技术研究肾上腺素能神经支配。下腹下神经去神经支配后，逼尿肌神经支配通常略有增加，三角区和尿道的肾上腺素能神经支配明显减少。副交感神经去神经支配后，逼尿肌的肾上腺素能神经支配增加，而三角区和尿道的神经支配无变化。副交感神经和下腹下神经同时去神经支配后，逼尿肌的肾上腺素能神经支配与仅行副交感神经去神经支配的膀胱相似。三角区和尿道的肾上腺素能神经支配与仅行下腹下神经去神经支配后的情况相似。采用一种记录不同药物处理后肌肉条等长张力变化的方法研究肾上腺素能受体功能。在正常状态下，去甲肾上腺素和去氧肾上腺素可引起三角区 - 尿道收缩，异丙肾上腺素则使其舒张。在逼尿肌，去甲肾上腺素可使由卡巴胆碱收缩的肌条舒张。松弛的逼尿肌肌条通常不会因去甲肾上腺素而收缩。然而，在8只正常猫中有2只，在给予非常大剂量的去甲肾上腺素后观察到了收缩。下腹下神经去神经支配后，肾上腺素能受体功能与正常状态一致。副交感神经去神经支配后，三角区 - 尿道受体功能与正常受体功能相比无变化。在逼尿肌，异丙肾上腺素可使其舒张，去甲肾上腺素和去氧肾上腺素则使其收缩。副交感神经和下腹下神经同时去神经支配后，结果与仅行副交感神经去神经支配后一致。因此，本研究表明，无论下腹下神经是否同时切断，副交感神经去神经支配后逼尿肌的肾上腺素能神经支配和α受体活性均增加。虽然正常逼尿肌中可能存在α受体，但β受体占主导地位。副交感神经去神经支配后，α受体和β受体之间的关系似乎发生了变化，有利于α受体。
