Okamoto M, Matsumoto T
Laboratory Animal and Plant Sciences, National Institute of Radiological Sciences, Chiba-shi, Japan.
Exp Anim. 1999 Jan;48(1):59-62. doi: 10.1538/expanim.48.59.
We applied the embryo transfer technique to germfree (GF) mouse production. Embryos harvested from superovulated mice were transferred aseptically, in a sterile environment, to the uterus of GF recipient females which had been mated with vasectomized GF males. One of the recipients became pregnant and delivered offspring. Sterility tests confirmed that the vasectomized males, newborns, recipient female mice, embryo-containing culture media, and the inside of the vinyl film isolator were germfree. These results suggest that the embryo transfer technique can be successfully applied to the production of GF mice.
我们将胚胎移植技术应用于无菌（GF）小鼠的生产。从超排卵小鼠采集的胚胎在无菌环境中无菌地移植到已与输精管结扎的GF雄性小鼠交配的GF受体雌性小鼠的子宫中。其中一只受体怀孕并产下后代。无菌测试证实，输精管结扎的雄性小鼠、新生小鼠、受体雌性小鼠、含胚胎的培养基以及乙烯基薄膜隔离器内部均无菌。这些结果表明，胚胎移植技术可成功应用于GF小鼠的生产。
