Chiba N, Iwasaki T, Mizutani T, Kariwa H, Kurata T, Takashima I
Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Vaccine. 1999 Feb 26;17(7-8):779-87. doi: 10.1016/s0264-410x(98)00262-x.
The pathogenic characteristics of tick-borne encephalitis (TBE) virus strain (Oshima 5-10) isolated from a sentinel dog in Hokkaido, Japan, was compared by use of a mouse model with several inoculation routes to other strains of TBE virus (the Far Eastern subtype; Sofjin strain and the Western subtype; Hochosterwitz strain) and TBE complex virus (Langat virus; TP-21 strain). The degree of neuroinvasiveness of the strains in mice subcutaneously (s.c.) inoculated was Sofjin equaled Hochosterwitz which was greater than Oshima and TP-21, respectively. Neurovirulence, as determined after intracerebral inoculation was Sofjin > Oshima = Hochosterwitz > TP-21. Virus replication in the brains of mice s.c. or intracerebrally inoculated with Oshima strain was slower and of lower titer than that of Sofjin strain. Histopathological findings indicated that subarachnoid infiltration of mononuclear cells prior to necrosis of the cerebrum was characteristic in Oshima strain. These findings indicated that the Oshima strain possessed a pathogenic potential common to TBE viruses and is less virulent for mice as compared with the two other TBE strains examined.
利用小鼠模型，通过几种接种途径，将从日本北海道一只哨兵犬分离出的蜱传脑炎（TBE）病毒株（大岛5 - 10）的致病特性与其他TBE病毒株（远东亚型；索夫金株和西方亚型；霍赫施特维茨株）以及TBE复合病毒（兰加特病毒；TP - 21株）进行了比较。皮下（s.c.）接种小鼠后，各毒株的神经侵袭程度为索夫金株等于霍赫施特维茨株，分别大于大岛株和TP - 21株。脑内接种后测定的神经毒力为索夫金株＞大岛株 = 霍赫施特维茨株＞TP - 21株。皮下或脑内接种大岛株的小鼠脑中病毒复制比索夫金株慢且滴度低。组织病理学结果表明，在大脑坏死之前蛛网膜下腔单核细胞浸润是大岛株的特征。这些结果表明，大岛株具有TBE病毒共有的致病潜力，与所检测的其他两种TBE毒株相比，对小鼠的毒力较低。
