Yamada T, Furukawa S, Hamazaki T, Songsri P
Department of Fermentation Technology, Faculty of Engineering, Hiroshima University, Japan.
Virology. 1996 May 15;219(2):395-406. doi: 10.1006/viro.1996.0265.
Several capsid proteins were selectively released from the viral core of Chlorella virus CVK2 by treatment with 4M urea. Among the viral core proteins, seven species (Vp154, Vp73, Vp63, Vp52, Vp48, Vp42, and Vp25) were shown to have DNA-binding activities by Southwestern blot analysis. Except for Vp154 and Vp25, these DNA-binding proteins showed a specific affinity for the viral genomic DNA. The viral core also contained three proteins with protein kinase activity (Vp73, Vp60, and Vp37); Vp73 seemed to have both DNA-binding and protein kinase activities. Antisera raised against Vp73 were used to screen a lambda-CVK2 expression library for the gene encoding Vp73. Three different clones (Vp73-3, Vp73-29, and Vp73-42) were obtained and analyzed. ORFs found in these clones all contained characteristic proline-rich motifs. The Vp73-42 ORF showed a strong similarity with histone H1 of various organisms and the Vp73-29 ORF contained two regions with leucine-zipper motifs. All three genes were expressed late in infection.
通过用4M尿素处理，几种衣壳蛋白从小球藻病毒CVK2的病毒核心中被选择性释放。在病毒核心蛋白中，通过蛋白质印迹分析显示七种蛋白（Vp154、Vp73、Vp63、Vp52、Vp48、Vp42和Vp25）具有DNA结合活性。除了Vp154和Vp25外，这些DNA结合蛋白对病毒基因组DNA表现出特异性亲和力。病毒核心还含有三种具有蛋白激酶活性的蛋白（Vp73、Vp60和Vp37）；Vp73似乎同时具有DNA结合和蛋白激酶活性。用针对Vp73产生的抗血清筛选λ-CVK2表达文库以寻找编码Vp73的基因。获得并分析了三个不同的克隆（Vp73-3、Vp73-29和Vp73-42）。在这些克隆中发现的开放阅读框都含有特征性的富含脯氨酸基序。Vp73-42开放阅读框与各种生物体的组蛋白H1有很强的相似性，Vp73-29开放阅读框含有两个带有亮氨酸拉链基序的区域。所有这三个基因在感染后期表达。
