Rostgaard J, Qvortrup K
Department of Medical Anatomy, The Panum Institute, University of Copenhagen, Denmark.
Acta Ophthalmol Scand. 1999 Feb;77(1):45-9. doi: 10.1034/j.1600-0420.1999.770111.x.
Production of reliable sections of fovea centralis of primate retina.
Rhesus monkey eyes were processed using a new technique known to minimize artefacts and cell-shrinkage in other tissues. An in vivo vascular perfusion-fixation technique with an isotonic fixative followed by a process of slow infiltration with small increments in concentrations were used.
Tangential 1 microm thick serial-sections showed a very good morphology. An average cone-to-cone-center distance of 3.8 microm in the central part of the fovea centralis was found. A cone-to-cone-center distance of 2.2-2.6 microm is suggested in the literature.
In spite of the limited study we believe that the new technique has resulted in less tissue shrinkage. Our results are discussed in relation to maximal possible resolution of the eye. Our calculations (for green light) support the idea that the structure of the fovea centralis and the refractive media of the eye are developed close to perfection.
制作灵长类动物视网膜中央凹的可靠切片。
采用一种已知可将其他组织中的伪像和细胞收缩降至最低的新技术处理恒河猴眼睛。使用等渗固定剂进行体内血管灌注固定技术，随后采用浓度逐步小幅增加的缓慢浸润过程。
1微米厚的连续切线切片显示出非常好的形态。在中央凹中心部分发现平均锥体细胞中心间距为3.8微米。文献中提出锥体细胞中心间距为2.2 - 2.6微米。
尽管研究有限，但我们认为新技术减少了组织收缩。我们的结果结合眼睛的最大可能分辨率进行了讨论。我们的计算（针对绿光）支持这样一种观点，即中央凹的结构和眼睛的屈光介质已发展得近乎完美。
