Bratteler Martin, Lexer Christian, Widmer Alex
ETH Zürich, Institute of Integrative Biology, Plan Ecological Genetics, 8092 Zürich, Switzerland.
Genome. 2006 Apr;49(4):320-7. doi: 10.1139/g05-114.
A genetic linkage map of an intraspecific cross between 2 Silene vulgaris s.l. ecotypes is presented. Three-hundred AFLP markers from 2 different restriction enzyme combinations were used to genotype an F2 mapping population. Maternal and paternal pure-coupling phase maps with 114 and 186 markers on 12 and 13 linkage groups, respectively, were constructed. Total map length of the paternal and maternal maps are 547 and 446 Kosambi cM, respectively. Nearly half of the markers (49%) exhibited significant transmission ratio distortion. Genome coverage and potential causes of the observed segregation ratio distortions are discussed. The maps represent a first step towards the identification of quantitative trait loci associated with habitat adaptation in the non-model species Silene vulgaris.
本文展示了两种广义蝇子草生态型种内杂交的遗传连锁图谱。使用来自2种不同限制酶组合的300个AFLP标记对一个F2作图群体进行基因分型。构建了母本和父本的纯合相图谱，分别在12个和13个连锁群上有114个和186个标记。父本和母本图谱的总长度分别为547和446 Kosambi cM。近一半的标记（49%）表现出显著的传递率畸变。讨论了基因组覆盖范围以及观察到的分离比畸变的潜在原因。这些图谱是朝着鉴定与非模式物种广义蝇子草栖息地适应性相关的数量性状基因座迈出的第一步。
