Lasota J, Miettinen M
Armed Forces Institute of Pathology, Department of Soft Tissue Pathology, Washington, DC 20306-6000, USA.
Virchows Arch. 1999 Jan;434(1):51-6. doi: 10.1007/s004280050304.
Kaposi's sarcoma-associated virus (human herpesvirus-8 [HHV8]) sequences have been identified in both AIDS-associated and AIDS-non-associated Kaposi's sarcoma, but data relating to the detection of HHV8 sequences in angiosarcoma are variable. One study showed HHV8 sequences in 29% (7/24) of angiosarcomas, but others have not confirmed these results. We evaluated 33 angiosarcomas for HHV8 sequences to determine the frequency of this virus in angiosarcomas and its possible pathogenetic significance. Five cases of Kaposi's sarcoma from HIV-positive patients were used as positive controls. Five additional cases of Kaposi's sarcoma collected approximately 40-50 years ago were also analysed, and three were HHV8 positive. None of the angiosarcomas revealed HHV8 sequences after the standard 35 cycles of PCR. In 6 cases, nested PCR revealed the presence of HHV8 sequences. These results were not reproducible when outer primers (based on sequences outside of the earlier PCR products) were used for amplification. This suggests that the HHV8 sequences detected in 6 cases represent a low level of contamination. In contrast, HHV8 sequences were found in all Kaposi's sarcomas with well-preserved DNA after standard 35-cycle PCR amplification. These findings confirm a close association between Kaposi's sarcoma and HHV8 infection and suggest that HHV8 is not involved in the pathogenesis of angiosarcoma.
卡波西肉瘤相关病毒（人类疱疹病毒8型[HHV8]）序列已在艾滋病相关和非艾滋病相关的卡波西肉瘤中被鉴定出来，但有关血管肉瘤中HHV8序列检测的数据并不一致。一项研究显示，29%（7/24）的血管肉瘤中有HHV8序列，但其他研究并未证实这些结果。我们评估了33例血管肉瘤中的HHV8序列，以确定该病毒在血管肉瘤中的频率及其可能的致病意义。5例来自HIV阳性患者的卡波西肉瘤病例用作阳性对照。还分析了另外5例大约在40 - 50年前收集的卡波西肉瘤病例，其中3例HHV8呈阳性。在标准的35个PCR循环后，所有血管肉瘤均未显示HHV8序列。在6例病例中，巢式PCR显示存在HHV8序列。当使用外部引物（基于早期PCR产物之外的序列）进行扩增时，这些结果无法重复。这表明在6例病例中检测到的HHV8序列代表低水平污染。相比之下，在标准的35个循环PCR扩增后，所有DNA保存完好的卡波西肉瘤中均发现了HHV8序列。这些发现证实了卡波西肉瘤与HHV8感染之间的密切关联，并表明HHV8不参与血管肉瘤的发病机制。
