McDonagh D P, Liu J, Gaffey M J, Layfield L J, Azumi N, Traweek S T
Department of Pathology, Duke University Medical Center, Durham, North Carolina 27710, USA.
Am J Pathol. 1996 Oct;149(4):1363-8.
The partial DNA sequence of a putative new herpesvirus has recently been isolated from almost all cases of Kaposi's sarcoma (KS), from a small subset of AIDS-related lymphomas, and from a high proportion of multicentric Castleman's disease. The presence of this KS-associated herpesvirus, which is also known as human herpes virus 8 (KSHV/ HHV8), has not been reported in vascular tumors other than KS. We therefore examined a series of vascular neoplasms of both endothelial and pericyte derivation using polymerase chain reaction to detect a 233-hp segment of the viral DNA. KSHV/HHV8 sequences were found in 7 of 24 (29%) angiosarcomas and 1 of 20 (5%) hemangiomas but not in any hemangiopericytomas (0 of 6). The presence of the virus in angiosarcoma was confirmed by direct sequencing of the polymerase chain reaction product and Southern blotting in one case each. Only one of the affected patients was known to be immunocompromised. By detecting its presence in a significant proportion of angiosarcomas, this study extends the number of tumors associated with KSHV/HHV8, further tightens its association with malignancy, and suggests a tropism of the virus for endothelial cells. The presence of KSHV/HHV8 in angiosarcomas in addition to classical KS also indicates that immunosuppression is not a requisite for viral infection.
一种假定的新型疱疹病毒的部分DNA序列最近已从几乎所有卡波西肉瘤（KS）病例、一小部分与艾滋病相关的淋巴瘤以及相当比例的多中心性Castleman病中分离出来。这种与KS相关的疱疹病毒，也被称为人类疱疹病毒8型（KSHV/HHV8），除KS外，尚未在其他血管肿瘤中报道。因此，我们使用聚合酶链反应检测病毒DNA的一个233个碱基对的片段，对一系列来源于内皮细胞和周细胞的血管肿瘤进行了检测。在24例血管肉瘤中有7例（29%）、20例血管瘤中有1例（5%）发现了KSHV/HHV8序列，但在任何血管外皮细胞瘤中均未发现（6例中0例）。通过对聚合酶链反应产物进行直接测序以及对其中1例进行Southern印迹分析，证实了血管肉瘤中存在该病毒。已知仅1例受影响患者存在免疫功能低下。通过在相当比例的血管肉瘤中检测到该病毒的存在，本研究扩展了与KSHV/HHV8相关的肿瘤数量，进一步加强了其与恶性肿瘤的关联，并提示该病毒对内皮细胞具有嗜性。除了经典的KS外，血管肉瘤中存在KSHV/HHV8还表明免疫抑制并非病毒感染的必要条件。
