Nakashima M, Toyono T, Akamine A, Joyner A
Department of Operative Dentistry and Endodontology, Faculty of Dentistry, Kyushu University, Fukuoka 812-82, Japan.
Mech Dev. 1999 Feb;80(2):185-9. doi: 10.1016/s0925-4773(98)00205-6.
We have cloned and characterized a new member of the bone morphogenetic protein/transforming growth factor beta (BMP/TGFbeta) superfamily, growth differentiation factor 11 (Gdf11), from rat incisor pulp RNA by reverse transcription-polymerase chain reaction using degenerate primers. The mature carboxyl-terminal domain encoded by Gdf11 is most closely related to Gdf8, being 90% identical to the mouse gene. Northern blot analysis revealed Gdf11 is expressed in adult dental pulp and brain. In situ hybridization of sections and whole-mount embryos demonstrated Gdf11 is first strongly expressed in restricted domains at 8.5 days post coitus (dpc) when it is highest in the tail bud. At 10.5 dpc, it is expressed in the branchial arches, limb bud, tail bud and posterior dorsal neural tube. Later, it is expressed in terminally-differentiated odontoblasts, the nasal epithelium, retina and specific regions of the brain.
我们通过使用简并引物进行逆转录-聚合酶链反应，从大鼠切牙牙髓RNA中克隆并鉴定了骨形态发生蛋白/转化生长因子β（BMP/TGFβ）超家族的一个新成员——生长分化因子11（Gdf11）。Gdf11编码的成熟羧基末端结构域与Gdf8关系最为密切，与小鼠基因的同一性为90%。Northern印迹分析显示Gdf11在成年牙髓和大脑中表达。切片和整装胚胎的原位杂交表明，Gdf11在交配后8.5天（dpc）时首先在特定区域强烈表达，此时在尾芽中表达量最高。在10.5 dpc时，它在鳃弓、肢芽、尾芽和后背部神经管中表达。之后，它在终末分化的成牙本质细胞、鼻上皮、视网膜和大脑的特定区域中表达。
