Ponassi M, Jacques T S, Ciani L, ffrench Constant C
Wellcome/CRC Institute of Developmental Biology and Cancer, Tennis Court Road, Cambridge, CB2 1QR, UK.
Mech Dev. 1999 Feb;80(2):207-12. doi: 10.1016/s0925-4773(98)00217-2.
We have sequenced and defined the expression during rat embryogenesis of the protocadherin fat, the murine homologue of a Drosophila tumour suppressor gene. As previously described for human fat, the sequence encodes a large protocadherin with 34 cadherin repeats, five epidermal growth factor (EGF)-like repeats containing a single laminin A-G domain and a putative transmembrane portion followed by a cytoplasmic sequence. This cytoplasmic sequence shows homology to the b-catenin binding regions of classical cadherin cytoplasmic tails and also ends with a PDZ domain-binding motif. In situ hybridization studies at E15 show that fat is predominately expressed in fetal epithelial cell layers and in the CNS, although expression is also seen in tongue musculature and condensing cartilage. Within the CNS, expression is seen in the germinal regions and in areas of developing cortex, and this neural expression pattern is also seen at later embryonic (E18) and postnatal stages. No labelling was seen in adult tissues except in the CNS, where the remnant of the germinal zones, as well as the dentate gyrus, continue to express fat.
我们已对原钙黏蛋白fat（果蝇肿瘤抑制基因的小鼠同源物）在大鼠胚胎发育过程中的表达进行了测序和定义。如先前对人fat的描述，该序列编码一种大型原钙黏蛋白，具有34个钙黏蛋白重复序列、五个包含单个层粘连蛋白A - G结构域的表皮生长因子（EGF）样重复序列、一个推定的跨膜部分，其后是一个细胞质序列。这个细胞质序列与经典钙黏蛋白细胞质尾部的β - 连环蛋白结合区域具有同源性，并且也以一个PDZ结构域结合基序结尾。在胚胎第15天（E15）进行的原位杂交研究表明，fat主要在胎儿上皮细胞层和中枢神经系统中表达，尽管在舌肌组织和凝聚的软骨中也可见表达。在中枢神经系统内，在生发区和发育中的皮质区域可见表达，并且这种神经表达模式在胚胎后期（E18）和出生后阶段也可见。除中枢神经系统外，在成年组织中未观察到标记，在中枢神经系统中，生发区残余以及齿状回继续表达fat。
