Lin H, Rao H
Department of Pathology, Sun Yat-sen University of Medical Sciences, Guangzhou.
Zhonghua Bing Li Xue Za Zhi. 1997 Aug;26(4):225-8.
To study the relationship between EB virus and benign malignant lymphoepithelial Lesions (ELEL) and malignant lymphoepithelial lesions (MLEL).
In situ hybridization, polymeras Chain reaction (PCR) and immunohistochemical methods were used to detect EBV DNA, EBER1 and latent membrane protein 1 (LMP1) on paraffin embedded tissues of 18 MLEL cases and 14 BLEL cases.
(1) Positive rates for Bam H1 W fragmet by PCR and EBER1 by in situ hybridization in 18 cases of MLEL were both 100%. Positive rates of EBV DNA by PCR and EBER1 by in situ hybridization in 14 cases of BLEL were both zero percent. (2) LMP1 expression was detected in 77.8% (14/18) of MLEL, EBNA2 was examined in 9 cases of MLEL and no expression was found. (3) Among the infiltrating lymphocytes studied by immunohistochemistry, T cells predominated over B cells in MLEL, B cells predominated over T cells in BLEL. (4) Of the 216 cases of salivary gland cancer, 37 cases could satisfy the criteria for MLEL. The incidence of MLEL in this group was 17.13% (37/216).
Our results suggest that EBV infection may have some relationship with the genesis of MLEL.
研究EB病毒与良性恶性淋巴上皮病变（ELEL）及恶性淋巴上皮病变（MLEL）之间的关系。
采用原位杂交、聚合酶链反应（PCR）及免疫组化方法，对18例MLEL病例和14例BLEL病例的石蜡包埋组织进行EBV DNA、EBER1及潜伏膜蛋白1（LMP1）检测。
（1）18例MLEL病例中，PCR检测Bam H1 W片段阳性率及原位杂交检测EBER1阳性率均为100%。14例BLEL病例中，PCR检测EBV DNA阳性率及原位杂交检测EBER1阳性率均为零。（2）18例MLEL中77.8%（14/18）检测到LMP1表达，9例MLEL检测EBNA2，未发现表达。（3）免疫组化研究浸润淋巴细胞中，MLEL中T细胞多于B细胞，BLEL中B细胞多于T细胞。（4）216例涎腺癌中，37例符合MLEL标准。该组MLEL发病率为17.13%（37/216）。
结果提示EBV感染可能与MLEL的发生有一定关系。
