甲苯咪唑和吡喹酮对小鼠体内细粒棘球绦虫囊肿壁中磷酸葡萄糖异构酶和甘油醛磷酸脱氢酶的影响。
Effect of mebendazole and praziquantel on glucosephosphate isomerase and glyceraldehydephosphate dehydrogenase in Echinococcus granulosus cyst wall harbored in mice.
作者信息
You J Q, Xiao S H, Guo H F, Jiao P Y, Mei J Y, Yao M Y
机构信息
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 Jan;18(1):75-7.
AIM
To study effects of antihydatid drugs on glucosephosphate isomerase (GPI) and glyceraldehydephosphate dehydrogenase (GAPDH) in Echinococcus granulosus cyst wall.
METHODS
Mice infected with the parasite for 8-10 months were treated i.g. with mebendazole (Meb) or praziquantel (Pra). The activities of GPI and GAPDH in the cysts were measured by the formation of NADH or NADPH.
RESULTS
GPI activity in the cyst wall was 197 +/- 103 U, while that of GAPDH was 25 +/- 13 U. When infected mice were treated i.g. with Meb 25-50 mg.kg-1.d-1 for 7-14 d, no apparent effect on the GAPDH activity in the cyst was found. In mice treated i.g. with praziquantel (Pra) 500 mg.kg-1.d-1 for 14 d, the GAPDH activity in the cyst wall was inhibited by 26.5%. As to GPI activity only the group treated i.g. with Meb 25 mg.kg-1.d-1 for 14 d showed 33.2% inhibition of the enzyme in the collapsed cyst wall.
CONCLUSION
GPI and GAPDH are not the major targets attacked by the antihydatid drug.
目的
研究抗包虫药物对细粒棘球绦虫囊壁中葡萄糖磷酸异构酶（GPI）和甘油醛磷酸脱氢酶（GAPDH）的影响。
方法
用甲苯达唑（Meb）或吡喹酮（Pra）灌胃治疗感染寄生虫8 - 10个月的小鼠。通过NADH或NADPH的生成来测定囊肿中GPI和GAPDH的活性。
结果
囊壁中GPI活性为197±103 U，而GAPDH活性为25±13 U。当感染小鼠用25 - 50 mg·kg-1·d-1的Meb灌胃治疗7 - 14天时，未发现对囊肿中GAPDH活性有明显影响。在用500 mg·kg-1·d-1的吡喹酮（Pra）灌胃治疗14天的小鼠中，囊壁中GAPDH活性被抑制了26.5%。对于GPI活性，只有用25 mg·kg-1·d-1的Meb灌胃治疗14天的组显示塌陷囊壁中的该酶被抑制了33.2%。
结论
GPI和GAPDH不是抗包虫药物攻击的主要靶点。
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