铜-阿司匹林复合物对血小板聚集的抑制作用。
Inhibitory effects of copper-aspirin complex on platelet aggregation.
作者信息
Shen Z Q, Chen Z H, Ma G Y, Wang D C, Wu W L, Liu W P, Yang Y K, Xiong H Z
机构信息
Yunnan Pharmacological Laboratories of Natural Products, Kunming Medical College, China.
出版信息
Zhongguo Yao Li Xue Bao. 1997 Jul;18(4):358-62.
AIM
To study the inhibitory effects of copper-aspirin complex (CuAsp) on platelet aggregation.
METHODS
With adenosine diphosphate the effects of CuAsp on platelet aggregation in vitro or in vivo were investigated. Radioimmunoassay and fluorophotometry were used to measure thromboxane B2 (TXB2) generation from platelets, the levels of TXB2 and of 6-keto-PGF1 alpha in plasma and the platelet serotonin release reaction.
RESULTS
In vitro, CuAsp inhibited arachidonic acid (AA)-induced aggregation (IC50 = 17 mumol.L-1, 95% confidence limits: 9-33 mumol.L-1), the release of 5-HT (IC50 = 19 mumol.L-1, 95% confidence limits: 10-30 mumol.L-1), and TXB2 generation from platelets (P < 0.05). CuAsp 10 mg.kg-1 i.g. selectively inhibited AA-induced aggregation, and increased the 6-keto-PGF1 alpha concentration in plasma while decreased that of TXB2.
CONCLUSION
CuAsp, in vitro or in vivo, shows more potent inhibitory effects on AA-induced aggregation than aspirin (Asp), related to the inhibition of platelet cyclooxygenase and the release of active substances from platelets.
目的
研究铜 - 阿司匹林复合物（CuAsp）对血小板聚集的抑制作用。
方法
采用二磷酸腺苷，研究CuAsp在体外或体内对血小板聚集的影响。运用放射免疫分析法和荧光光度法测定血小板血栓素B2（TXB2）的生成、血浆中TXB2和6 - 酮 - 前列腺素F1α的水平以及血小板5 - 羟色胺释放反应。
结果
在体外，CuAsp抑制花生四烯酸（AA）诱导的聚集（IC50 = 17 μmol·L-1，95%置信限：9 - 33 μmol·L-1）、5 - 羟色胺（5 - HT）的释放（IC50 = 19 μmol·L-1，95%置信限：10 - 30 μmol·L-1）以及血小板TXB2的生成（P < 0.05）。灌胃给予10 mg·kg-1的CuAsp可选择性抑制AA诱导的聚集，并使血浆中6 - 酮 - 前列腺素F1α浓度升高，同时降低TXB2浓度。
结论
CuAsp在体外或体内对AA诱导的聚集显示出比阿司匹林（Asp）更强的抑制作用，这与抑制血小板环氧化酶以及血小板活性物质的释放有关。
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