一个错义突变导致了plsB26突变体中表达的甘油-3-磷酸酰基转移酶的缺陷。
A missense mutation accounts for the defect in the glycerol-3-phosphate acyltransferase expressed in the plsB26 mutant.
作者信息
Heath R J, Rock C O
机构信息
Department of Biochemistry, St Jude Children's Research Hospital, Memphis, Tennessee 38101, USA.
出版信息
J Bacteriol. 1999 Mar;181(6):1944-6. doi: 10.1128/JB.181.6.1944-1946.1999.
Abstract
The sn-glycerol-3-phosphate acyltransferase (plsB) catalyzes the first step in membrane phospholipid formation. A conditional Escherichia coli mutant (plsB26) has a single missense mutation (G1045A) predicting the expression of an acyltransferase with an Ala349Thr substitution. The PlsB26 protein had a significantly reduced glycerol-3-phosphate acyltransferase specific activity coupled with an elevated Km for glycerol-3-phosphate.
摘要
sn-甘油-3-磷酸酰基转移酶（plsB）催化膜磷脂形成的第一步。一个条件性大肠杆菌突变体（plsB26）有一个单一的错义突变（G1045A），预测表达一种具有Ala349Thr替代的酰基转移酶。PlsB26蛋白的甘油-3-磷酸酰基转移酶比活性显著降低，同时对甘油-3-磷酸的Km升高。
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本文引用的文献
1
A conserved histidine is essential for glycerolipid acyltransferase catalysis.一个保守的组氨酸对于甘油脂酰基转移酶催化作用至关重要。
J Bacteriol. 1998 Mar;180(6):1425-30. doi: 10.1128/JB.180.6.1425-1430.1998.
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A gene (plsD) from Clostridium butyricum that functionally substitutes for the sn-glycerol-3-phosphate acyltransferase gene (plsB) of Escherichia coli.来自丁酸梭菌的一个基因（plsD），其在功能上可替代大肠杆菌的sn-甘油-3-磷酸酰基转移酶基因（plsB）。
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