膜磷脂合成存在缺陷的大肠杆菌突变体：sn-甘油-3-磷酸酰基转移酶Km突变体的定位
Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of sn-glycerol 3-phosphate acyltransferase Km mutants.
作者信息
Cronan J E, Bell R M
出版信息
J Bacteriol. 1974 Oct;120(1):227-33. doi: 10.1128/jb.120.1.227-233.1974.
Abstract
plsB mutants of Escherichia coli are sn-glycerol 3-phosphate auxotrophs which owe their requirement to a K(m) defect in sn-glycerol 3-phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. We have located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene defined by mutants with a temperature-sensitive sn-glycerol 3-phosphate acyltransferase. The plsB gene was cotransduced with the dctA locus, and the transduction data indicated that the clockwise gene order is asd, plsB, dctA, xyl. plsB(-) is recessive to plsB(+) and all acyltransferase K(m) mutants tested lie very close to the plsB locus. Effective supplementation of plsB mutants was shown not to require a defective glpD gene.
摘要
大肠杆菌的plsB突变体是sn-甘油3-磷酸营养缺陷型，其对sn-甘油3-磷酸的需求归因于磷脂生物合成途径中的第一种酶——sn-甘油3-磷酸酰基转移酶的Km缺陷。我们已将plsB基因定位在大肠杆菌遗传图谱的69分钟处，与具有温度敏感型sn-甘油3-磷酸酰基转移酶的突变体所定义的基因相距甚远。plsB基因与dctA位点共转导，转导数据表明顺时针基因顺序为asd、plsB、dctA、xyl。plsB(-)对plsB(+)是隐性的，并且所有测试的酰基转移酶Km突变体都非常靠近plsB位点。结果表明，有效补充plsB突变体不需要有缺陷的glpD基因。
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