Lawson P A, Collins M D, Falsen E, Sjöden B, Facklam R R
Department of Food Science and Technology, University of Reading, Reading, United Kingdom.
J Clin Microbiol. 1999 Apr;37(4):1161-4. doi: 10.1128/JCM.37.4.1161-1164.1999.
Three strains of a gram-positive catalase-negative, facultatively anaerobic coccus-shaped organism originating from human clinical samples were characterized by phenotypic and molecular taxonomic methods. Sequencing of genes encoding 16S rRNA showed that the strains are phylogenetically closely related (99.9 to 100% sequence similarity) and represent a new subline within the genus Facklamia. The unknown bacterium was readily distinguished from all currently described species of the genus Facklamia (viz., Facklamia hominis, Facklamia ignava, and Facklamia sourekii) by biochemical tests and electrophoretic analysis of whole-cell proteins. Based on phylogenetic and phenotypic evidence, it is proposed that the unknown bacterium be classified as Facklamia languida sp. nov. The type strain of F. languida is CCUG 37842.
采用表型和分子分类学方法对3株源自人类临床样本的革兰氏阳性、过氧化氢酶阴性、兼性厌氧的球菌状微生物进行了鉴定。编码16S rRNA的基因测序表明，这些菌株在系统发育上密切相关（序列相似性为99.9%至100%），代表了Facklamia属内的一个新亚系。通过生化试验和全细胞蛋白的电泳分析，该未知细菌很容易与Facklamia属目前描述的所有物种（即人Facklamia、慵懒Facklamia和索氏Facklamia）区分开来。基于系统发育和表型证据，建议将该未知细菌分类为慵懒Facklamia菌新种。慵懒Facklamia的模式菌株为CCUG 37842。
