Chavrier C A, Couble M L
Department of Biologic Sciences, School of Dentistry, University Claude Bernard, Lyon, France.
Int J Oral Maxillofac Implants. 1999 Jan-Feb;14(1):108-12.
The aim of this study was to investigate the ultrastructural and immunohistochemical organization of the main collagenous components of healthy human keratinized mucosa surrounding endosseous implants. Eight patients with completely edentulous mandibles were selected. Four endosseous implants were placed in the mandible of each patient, connected with a bar to support a complete overdenture, and loaded 4 months later. Two years after placement, biopsies of surrounding soft tissue, including the sulcular and junctional epithelium with the underlying and supracrestal connective tissue, were routinely prepared for standard electron microscopy and for ultrastructural immunolabeling of Types I, III, and IV collagen. The connective tissue located under the junctional epithelium comprised Types I and III collagen, whereas the supracrestal connective tissue was composed mainly of Type I collagen. Type IV collagen was located exclusively in the basement membrane of the junctional epithelium.
本研究的目的是调查围绕骨内种植体的健康人角化黏膜主要胶原成分的超微结构和免疫组织化学结构。选取了8例下颌完全无牙的患者。在每位患者的下颌骨中植入4枚骨内种植体，用杆连接以支持全口覆盖义齿，并在4个月后加载。植入后两年，对周围软组织进行活检，包括龈沟上皮和结合上皮及其下方和嵴上结缔组织，常规制备用于标准电子显微镜检查以及I型、III型和IV型胶原的超微结构免疫标记。位于结合上皮下方的结缔组织包含I型和III型胶原，而嵴上结缔组织主要由I型胶原组成。IV型胶原仅位于结合上皮的基底膜中。
