Bruch J, Wöhr G, Brüderlein S, Barbi G, Wolter H, Dixkens C, Mattfeldt T, Möller P, Paiss T, Hautmann R, Vogel W, Hameister H
Department of Medical Genetics, University of Ulm, Germany.
Int J Cancer. 1999 Mar 15;80(6):903-10. doi: 10.1002/(sici)1097-0215(19990315)80:6<903::aid-ijc17>3.0.co;2-8.
A permanent cell line, U-BLC1, was established from a primary transitional-cell carcinoma, TCC, of the urinary bladder. Karyotype analysis showed the line to be highly aberrant, with a near-triploid chromosome number of 68 to 73. Comparative genomic hybridization revealed some distinct differences between the primary tumor and the established cell line. Karyotype analysis showed 3 marker chromosomes with homogeneously staining regions, HSRs, in the cell line. The HSRs were isolated by microdissection and the microdissection probes were hybridized to normal metaphase chromosomes. The HSRs contain sequences known to be frequently involved in amplification in transitional-cell carcinoma of the bladder, 6p22, 7p11-p12, 9p23-pter, and one region not yet reported to be amplified in primary TCC of the bladder, 1p31-p32. A candidate-gene approach showed that in the region 7p11-p12 the EGFR locus is amplified and highly expressed.
一个永久性细胞系U - BLC1是从膀胱原发性移行细胞癌（TCC）建立的。核型分析表明该细胞系高度异常，染色体数接近三倍体，为68至73条。比较基因组杂交显示原发性肿瘤与建立的细胞系之间存在一些明显差异。核型分析显示该细胞系中有3条带有均匀染色区（HSRs）的标记染色体。通过显微切割分离出HSRs，并将显微切割探针与正常中期染色体杂交。HSRs包含已知在膀胱移行细胞癌中频繁参与扩增的序列，6p22、7p11 - p12、9p23 - pter，以及一个尚未报道在膀胱原发性TCC中扩增的区域，1p31 - p32。候选基因方法表明在7p11 - p12区域，表皮生长因子受体（EGFR）基因座被扩增并高表达。
