幽门螺杆菌感染诱导的细胞凋亡在十二指肠溃疡发生中的作用
Role of apoptosis induced by Helicobacter pylori infection in the development of duodenal ulcer.
作者信息
Kohda K, Tanaka K, Aiba Y, Yasuda M, Miwa T, Koga Y
机构信息
Department of Internal Medicine VI, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
出版信息
Gut. 1999 Apr;44(4):456-62. doi: 10.1136/gut.44.4.456.
BACKGROUND
Helicobacter pylori affects gastric epithelium integrity by acceleration of apoptosis. However, it remains unclear what product of the bacteria causes apoptosis, or whether or not the apoptosis is involved in the development of ulcers.
AIMS
To elucidate the factor from H pylori that causes acceleration of apoptosis and the role of apoptosis in the development of duodenal ulcer in H pylori infection.
PATIENTS
Five H pylori negative healthy volunteers, 47 H pylori positive patients with duodenal ulcer, and 35 H pylori positive patients with gastric ulcer.
METHODS
An endoscopic examination was carried out to diagnose ulcers and determine their clinical stage. To analyse apoptosis, a cell cycle analysis was performed using biopsy specimens.
RESULTS
There was a significant correlation between the urease activity of the H pylori strain and the level of apoptosis induced by this bacterial strain. Moreover, in duodenal ulcer patients infected with H pylori, the patients with an active ulcer exhibited a significantly higher level of apoptosis than those with ulcers at both the healing and scarring stages.
CONCLUSION
These findings suggest that acceleration of apoptosis in the antral mucosa caused by the urease of H pylori plays a crucial role in the development of ulcers in the duodenum.
背景
幽门螺杆菌通过加速细胞凋亡影响胃上皮完整性。然而，尚不清楚该细菌的何种产物导致细胞凋亡，以及细胞凋亡是否参与溃疡的发生发展。
目的
阐明幽门螺杆菌中导致细胞凋亡加速的因素以及细胞凋亡在幽门螺杆菌感染所致十二指肠溃疡发生发展中的作用。
患者
5名幽门螺杆菌阴性健康志愿者、47名幽门螺杆菌阳性十二指肠溃疡患者以及35名幽门螺杆菌阳性胃溃疡患者。
方法
进行内镜检查以诊断溃疡并确定其临床分期。为分析细胞凋亡，使用活检标本进行细胞周期分析。
结果
幽门螺杆菌菌株的尿素酶活性与该菌株诱导的细胞凋亡水平之间存在显著相关性。此外，在感染幽门螺杆菌的十二指肠溃疡患者中，活动期溃疡患者的细胞凋亡水平显著高于愈合期和瘢痕期溃疡患者。
结论
这些发现表明，幽门螺杆菌尿素酶引起的胃窦黏膜细胞凋亡加速在十二指肠溃疡的发生发展中起关键作用。
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