Savolainen H, Vainio H
Acta Neuropathol. 1976 Nov 15;36(3):251-7. doi: 10.1007/BF00685369.
The binding of carbon disulphide sulphur was studied in the spinal cord and its axons of four control and four phenobarbitone pretreated adult rats 3 and 6 h after an intraperitoneal injection of 650 mumol of CS2 in olive oil. The binding of CS2 carbon was measured in the same fractions of two adult control rats 4.5 h after a similar administration of 1.3 mumol of the compound for reference. The specific binding of sulphur was highest in the axons of control animals 3 h after the injection while binding was 17.5% smaller in the axons of phenobarbitone treated animals. The uptake of sulphur was higher in the spinal cord homogenate of the pretreated animals in comparison to control rats. Sulphur was removed from the axonal fraction at a rate of 5.1 natoms/mg of protein/h. Phenobarbitone treatment increased the disappearance of sulphur in the control animals while the rate in the treated rats was 4.9 natoms/mg of protein/h. Phenobarbitone treatment increased the disappearance of sulphur in the spinal homogenate from the removal rate of 0.22 natoms/mg of protein/h in control animals to 0.37 natoms/mg of protein/h in treated rats. The binding of CS2 carbon was negligible 4.5 h after the injection in the axonal fraction. The present data indicate that the release and binding of CS2 sulphur may be responsible for the toxic neural manifestations in chronic CS2 poisoning.
在腹腔注射650微摩尔溶解于橄榄油中的二硫化碳（CS2）3小时和6小时后，对4只对照成年大鼠和4只经苯巴比妥预处理的成年大鼠的脊髓及其轴突中二硫化碳硫的结合情况进行了研究。为作参考，在对2只成年对照大鼠以同样方式给予1.3微摩尔该化合物4.5小时后，对相同组分中二硫化碳碳的结合情况进行了测定。注射后3小时，对照动物轴突中硫的特异性结合最高，而经苯巴比妥处理动物的轴突中结合量则少17.5%。与对照大鼠相比，预处理动物脊髓匀浆中硫的摄取更高。硫从轴突组分中以5.1纳原子/毫克蛋白质/小时的速率被去除。苯巴比妥处理增加了对照动物中硫的消失量，而处理后大鼠中的速率为4.9纳原子/毫克蛋白质/小时。苯巴比妥处理使脊髓匀浆中硫的消失量从对照动物的0.22纳原子/毫克蛋白质/小时的去除率增加到处理后大鼠的0.37纳原子/毫克蛋白质/小时。注射后4.5小时，轴突组分中二硫化碳碳的结合可忽略不计。目前的数据表明，二硫化碳硫的释放和结合可能是慢性二硫化碳中毒时神经毒性表现的原因。
